Inhibition of HIV-1 replication by chimeric phosphorothioate oligodeoxynucleotides applied in free solution

被引：0
|
作者：
Lund, OS [1 ]
Hansen, JES [1 ]
机构：
[1] Univ Copenhagen, Hvidovre Hosp, Infect Dis Lab, Dept 144, DK-2650 Hvidovre, Denmark
来源：
INTERVIROLOGY | 1998年 / 41卷 / 2-3期
关键词：
human immunodeficiency virus; phosphorothioate; chimeric oligodeoxynucleotides; acute infection;
D O I：
10.1159/000024916
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Oligodeoxynucleotides (ODNs) containing a variable number of 3' and 5' terminal phosphorothioate linkages were applied in free solution to cells infected by HIV-1. ODNs of 28 nt length were applied at up to 5 mu M concentration. The ODNs were found to inhibit HIV-1 infection in a dose dependent manner, which correlated with the number of modified linkages (4, 8 and 12, respectively). A target sequence in the HIV-1 rev mRNA, previously reported as sensitive to antisense inhibition by full length phosphorothioate ODNs, only revealed non-sequence dependent inhibition of HIV-1, when tested by these modified chimers.
引用
收藏
页码：63 / 68
页数：6
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