Mucilaginibacter hurinus sp. nov., isolated from briquette warehouse soil

A novel bacterial strain, designated ZR32(T), was isolated from briquette warehouse soil in Ulsan (Korea). The strain was aerobic, showing pink-colored colonies on R2A agar. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain ZR32(T) was closely related to Mucilaginibacter soli R9-65(T) (97.0%), Mucilaginibacter gynuensis YC7003(T) (96.9%), and Mucilaginibacter lutimaris BR-3(T) (96.8%). The values of DNA-DNA relatedness related two highest strains M. soli R9-65(T) and M. gynuensis YC7003(T) were 31.2 +/- 6.9% and 19.7 +/- 0.3%, respectively. Its genome size was 3.9 Mb, comprising 3402 predicted genes. The DNA G+C content of strain ZR32(T) was 43.0 mol%. The major cellular fatty acids (> 5% of total) were summed feature 3 (C-16:1 omega 6c and/or C-16:1 omega 7c), C-16:0, C-16:1 omega 5c, iso-C-15:0, iso-C-17:0 3-OH, and C-17:1 omega 9c. The major respiratory quinine was menaquinone-7 (MK-7). The major polar lipids were phosphatidylethanolamine, two unidentified phospholipids, one unidentified sphingolipid, and one unidentified polar lipid. Strain ZR32(T) showed distinctive characteristics such as the temperature and pH for growth ranges, being positive for beta-glucosidase, salicin production, negative for N-acetyl-glucosamine assimilation, being resistant to carbenicillin and piperacillin to related species. On the basis of phenotypic, chemotaxonomic, and phylogenetic data, strain ZR32(T) represents a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter hurinus sp. nov. is proposed. The type strain is ZR32(T) (= KCTC 62193 = CCTCC AB 2017285).