Spatial distribution of manganese in enamel and coronal dentine of human primary teeth

被引:67
作者
Arora, Manish [1 ,2 ,3 ,4 ]
Hare, Dominic [3 ]
Austin, Christine [3 ]
Smith, Donald R. [4 ]
Doble, Philip [3 ]
机构
[1] Westmead Hosp, Inst Dent Res, Westmead Ctr Oral Hlth, Westmead, NSW 2145, Australia
[2] Univ Sydney, Fac Dent, Sydney, NSW 2006, Australia
[3] Univ Technol Sydney, Dept Chem & Forens Sci, Elemental Bioimaging Facil, Sydney, NSW 2007, Australia
[4] Univ Calif Santa Cruz, Dept Environm Toxicol, Santa Cruz, CA 95064 USA
关键词
Bio-imaging; Dentine; Enamel; Laser ablation; Manganese; BONE LEAD; EXPOSURE; AGE; BANGLADESH; BIOMARKER; CALCIUM; WATER;
D O I
10.1016/j.scitotenv.2010.12.018
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Emerging evidence indicates that excessive exposure to manganese (Mn) during the prenatal period and early childhood may result in neurodevelopmental deficits. However, accurate exposure biomarkers are not well established, limiting our understanding of exposure-response relationships over these susceptible periods of development. Naturally shed deciduous teeth are potentially a useful biomarker of environmental exposure to Mn. However, the uptake and distribution of Mn in human teeth has not been studied in detail. Mn distribution was measured at high resolution (similar to 20 mu m) in eight human primary teeth using laser ablation-inductively coupled plasma-mass spectrometry. A bio-imaging methodology was applied to construct detailed elemental maps of three incisors, and bone meal (NIST SRM 1486) was used to validate the analyses. The distribution of Mn in enamel and coronal dentine showed a distinct and reproducible pattern. In enamel, the (55)Mn:(43)Ca ratio was highest at the outer edge of enamel (range=0.57 to 4.74) for approximately 20-40 mu m but was substantially lower in deeper layers (range=0.005 to 0.013). The highest levels of Mn were observed in dentine immediately adjacent the pulpal margin ((55)Mn:(43)Ca range=2.27 to 6.95). Importantly, a clearly demarcated high Mn zone was observed in dentine at the incisal end of the teeth. Using confocal laser scanning microscopy to visualize the neonatal line, this region was identified as being in the prenatally formed dentine. The high-resolution map of the spatial distribution of Mn in human primary teeth highlighted specific reproducible patterns of Mn distribution in enamel and coronal dentine. (C) 2010 Elsevier B.V. All rights reserved.
引用
收藏
页码:1315 / 1319
页数:5
相关论文
共 28 条
[1]  
Arai K, 1989, Shoni Shikagaku Zasshi, V27, P487
[2]   Spatial distribution of lead in enamel and coronal dentine of Wistar rats [J].
Arora, M ;
Chan, SWY ;
Ryan, CG ;
Kennedy, BJ ;
Walker, DM .
BIOLOGICAL TRACE ELEMENT RESEARCH, 2005, 105 (1-3) :159-170
[3]  
ATSDR, 2008, DRAFT TOX PROF MANG
[4]   Elemental bio-imaging of calcium phosphate crystal deposits in knee samples from arthritic patients [J].
Austin, Christine ;
Hare, Dominic ;
Rozelle, Andrew L. ;
Robinson, William H. ;
Grimm, Rudolf ;
Doble, Philip .
METALLOMICS, 2009, 1 (02) :142-147
[5]   ATTENTIONAL CORRELATES OF DENTIN AND BONE LEAD LEVELS IN ADOLESCENTS [J].
BELLINGER, D ;
HU, H ;
TITLEBAUM, L ;
NEEDLEMAN, HL .
ARCHIVES OF ENVIRONMENTAL HEALTH, 1994, 49 (02) :98-105
[6]  
Berkovitz B.K., 2009, ORAL ANATOMY HISTOLO
[7]   Hair manganese and hyperactive behaviors: Pilot study of school-age children exposed through tap water [J].
Bouchard, Maryse ;
Laforest, Francois ;
Vandelac, Louise ;
Bellinger, David ;
Mergler, Donna .
ENVIRONMENTAL HEALTH PERSPECTIVES, 2007, 115 (01) :122-127
[8]   The distribution of lead within ancient and modern human teeth: Implications for long-term and historical exposure monitoring [J].
Budd, P ;
Montgomery, J ;
Cox, A ;
Krause, P ;
Barreiro, B ;
Thomas, RG .
SCIENCE OF THE TOTAL ENVIRONMENT, 1998, 220 (2-3) :121-136
[9]   Measurements of manganese with respect to calcium in histological enamel cross sections: Toward a new manganese biomarker [J].
Ericson, JE ;
Rinderknecht, A ;
Gonzalez, EJ ;
Crinella, FM ;
Kleinman, MT .
ENVIRONMENTAL RESEARCH, 2001, 86 (01) :46-50
[10]  
Frausto da Silva J. R., 2001, BIOL CHEM ELEMENTS I