Biological Cluster Mass Spectrometry

被引：56

作者：

Winograd, Nicholas ^{[1
]}

Garrison, Barbara J. ^{[1
]}

机构：

[1] Penn State Univ, Dept Chem, University Pk, PA 16802 USA

来源：

ANNUAL REVIEW OF PHYSICAL CHEMISTRY, VOL 61 | 2010年 / 61卷

基金：

美国国家科学基金会;

关键词：

secondary ion mass spectrometry; bioimaging; molecular depth profiling; three-dimensional molecular imaging; C(60); molecular dynamics; MOLECULAR-DYNAMICS SIMULATIONS; TOF-SIMS; ION-BEAMS; ENERGY DEPOSITION; SPUTTERING YIELDS; GA BOMBARDMENT; C-60; MODEL; SURFACES; BENZENE;

D O I：

10.1146/annurev.physchem.040808.090249

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

This article reviews the new physics and new applications of secondary ion mass spectrometry using cluster ion probes. These probes, particularly C(60), exhibit enhanced molecular desorption with improved sensitivity owing to the unique nature of the energy-deposition process. In addition, these projectiles are capable of eroding molecular solids while retaining the molecular specificity of mass spectrometry. When the beams are microfocused to a spot on the sample, bioimaging experiments in two and three dimensions are feasible. We describe emerging theoretical models that allow the energy-deposition process to be understood on an atomic and molecular basis. Moreover, experiments on model systems are described that allow protocols for imaging on biological materials to be implemented. Finally, we present recent applications of imaging to biological tissue and single cells to illustrate the future directions of this methodology.

引用

页码：305 / 322

页数：18