Molecular characterization of a new fish specific chemokine CXCL_F6 in large yellow croaker (Larimichthys crocea) and its role in inflammatory response

被引:14
|
作者
Mu, Yinnan [1 ]
Zhou, Shimin [1 ]
Ding, Ning [1 ]
Ao, Jingqun [3 ]
Chen, Xinhua [1 ,2 ]
机构
[1] Fujian Agr & Forestry Univ, Coll Anim Sci, Inst Oceanol, Fuzhou 350002, Fujian, Peoples R China
[2] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
[3] State Ocean Adm, Key Lab Marine Biogenet Resources, Inst Oceanog 3, Xiamen 361005, Peoples R China
基金
中国国家自然科学基金;
关键词
CXC chemokine; CXCL_F6; Chemotaxis; Large yellow croaker (Larimichthys crocea); Inflammatory response; NITRIC-OXIDE SYNTHASE; CXC CHEMOKINES; EXPRESSION ANALYSIS; INTERFERON-GAMMA; GENE; REVEALS; DEFENSE; MOTIF;
D O I
10.1016/j.fsi.2018.10.068
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
Chemokines are a superfamily of structurally related chemotactic cytokines exerting significant roles in regulating cell migration and activation. Currently, five subgroups of fish specific CXC chemokines, named CXCL_F1-CXCL_F5, have been identified in teleost fish. However, understanding of the functions of these fish specific CXC chemokines is still limited. Here, a new member of fish specific CXC chemokines, LcCXCL_F6, was cloned from large yellow croaker Larimichthys crocea. Its open reading frame (ORF) is 369 nucleotides long, encoding a peptide of 122 amino acids (aa). The deduced LcCXCL_F6 protein contains a 19-aa signal peptide and a 103-aa mature polypeptide, which has four conserved cysteine residues (C-28, C-30, C-88, and C-72), as found in other known CXC chemokines. Phylogenetic analysis showed LcCXCL_F6 formed a separate Glade with sequences from other fish species, tentatively named CXCL_F6, distinct from the clades formed by fish OCCL_F1-5 and mammalian CXC chemokines. The LcCXCL_F6 transcripts were constitutively expressed in all examined tissues and significantly up-regulated in the spleen and head kidney tissues by poly (I:C) and Vibrio alginolyticus. Its transcripts were also detected in primary head kidney leukocytes (HKLs), peripheral blood leucocytes (PBLs), and large yellow croaker head kidney (LYCK) cell line, and significantly up-regulated by poly(I:C), lipopolysaccharide (LPS), and peptidoglycan (PGN) in HKLs. Recombinant LcCXCL_F6 protein (rLcCXCL_F6) could not only chemotactically attract monocytes/macrophages and lymphocytes from PBLs, but also enhance NO release and expression of proinflammatory cytokines (TNF-alpha, IL-1 beta, and CXCL8) in monocytes/macrophages. These results indicate that LcCXCL_F6 plays a role in mediating the inflammatory response.
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页码:787 / 794
页数:8
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