Four subunit a isoforms of Caenorhabditis elegans vacuolar H+-ATPase -: Cell-specific expression during development

被引:106
|
作者
Oka, T
Toyomura, T
Honjo, K
Wada, Y
Futai, M
机构
[1] Osaka Univ, Inst Sci & Ind Res, Div Biol Sci, Ibaraki, Osaka 5670047, Japan
[2] Japan Sci & Technol Corp, Core Res Evolut Sci & Technol, Ibaraki, Osaka 5670047, Japan
关键词
D O I
10.1074/jbc.M101652200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have identified four genes (vha-5, vha-6, vha-7, and unc-32) coding for vacuolar-type proton-translocating ATPase (V-ATPase) subunit a in Caenorhabditis elegans, the first example of four distinct isoforms in eukaryotes. Their products had nine putative transmembrane regions, exhibited 43-60% identity and 62-84% similarity with the bovine subunit al isoform, and retained 11 amino acid residues essential for yeast V-ATPase activity (Leng, X. H., Manolson, M. F., and Forgac, M. (1998) J. Biol. Chem. 273, 6717-6723). The similarities, together with the results of immunoprecipitation, suggest that these isoforms are components of V-ATPase. Transgenic and immunofluorescence analyses revealed that these genes were strongly expressed in distinct cells; vha-5 was strongly expressed in an H-shaped excretory cell, vha-6 was strongly expressed in intestine, vha-7 was strongly expressed in hypodermis, and unc-32 was strongly expressed in nerve cells. Furthermore, the vha-7 and unc-32 genes were also expressed in the uteri of hermaphrodites. RNA interference analysis showed that the double-stranded RNA for unc-32 caused embryonic lethality similar to that seen with other subunit genes (vha-1, vha-4, and vha-11) (Oka, T., and Futai, M. (2000) J. Biol. Chem. 275, 29556-29561). The progenies of worms injected with the vha-5 or vha-6 double-stranded RNA became died at a specific larval stage, whereas the vha-7 double-stranded RNA showed no effect on development. These results suggest that V-ATPases with these isoforms generate acidic compartments essential for worm development in a cell-specific manner.
引用
收藏
页码:33079 / 33085
页数:7
相关论文
共 50 条
  • [1] Vacuolar-type H+-ATPase E subunit is required for embryogenesis and yolk transfer in Caenorhabditis elegans
    Choi, KY
    Ji, YJ
    Dhakal, BK
    Yu, JR
    Cho, C
    Song, WK
    Ahnn, J
    GENE, 2003, 311 : 13 - 23
  • [2] IDENTIFICATION OF 2 SUBUNIT A ISOFORMS OF THE VACUOLAR H+-ATPASE IN HUMAN OSTEOCLASTOMA
    VANHILLE, B
    RICHENER, H
    EVANS, DB
    GREEN, JR
    BILBE, G
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1993, 268 (10) : 7075 - 7080
  • [3] HETEROGENEITY OF VACUOLAR H+-ATPASE - DIFFERENTIAL EXPRESSION OF 2 HUMAN SUBUNIT B-ISOFORMS
    VANHILLE, B
    RICHENER, H
    SCHMID, P
    PUETTNER, I
    GREEN, JR
    BILBE, G
    BIOCHEMICAL JOURNAL, 1994, 303 : 191 - 198
  • [4] Three subunit a isoforms of mouse vacuolar H+-ATPase -: Preferential expression of the a3 isoform during osteoclast differentiation
    Toyomura, T
    Oka, T
    Yamaguchi, C
    Wada, Y
    Futai, M
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (12) : 8760 - 8765
  • [5] Tissue specificity of E subunit isoforms of plant vacuolar H+-ATPase and existence of isotype enzymes
    Kawamura, Y
    Arakawa, K
    Maeshima, M
    Yoshida, S
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (09) : 6515 - 6522
  • [6] Temporal control of cell-specific transgene expression in Caenorhabditis elegans
    Bacaj, Taulant
    Shaham, Shai
    GENETICS, 2007, 176 (04) : 2651 - 2655
  • [7] α4, a unique kidney-specific isoform of mouse vacuolar H+-ATPase subunit a
    Oka, T
    Murata, Y
    Namba, M
    Yoshimizu, T
    Toyomura, T
    Yamamoto, A
    Sun-Wada, GH
    Hamasaki, N
    Wada, Y
    Futai, M
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (43) : 40050 - 40054
  • [8] N-linked glycosylation of a subunit isoforms is critical for vertebrate vacuolar H+-ATPase (V-ATPase) biosynthesis
    Esmail, Sally
    Kartner, Norbert
    Yao, Yeqi
    Kim, Joo Wan
    Reithmeier, Reinhart A. F.
    Manolson, Morris F.
    JOURNAL OF CELLULAR BIOCHEMISTRY, 2018, 119 (01) : 861 - 875
  • [9] Identification of a vacuolar type H+-ATPase subunit A cDNA and three putative subunit B isoforms in Arabidopsis thaliana.
    Magnotta, S
    Gogarten, JP
    PLANT PHYSIOLOGY, 1997, 114 (03) : 972 - 972
  • [10] DIFFERENTIAL EXPRESSION OF THE B-SUBUNIT OF THE VACUOLAR H+-ATPASE IN BOVINE-TISSUES
    PUOPOLO, K
    KUMAMOTO, C
    ADACHI, I
    MAGNER, R
    FORGAC, M
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1992, 267 (06) : 3696 - 3706