DNA Damage Products (5′R)- and (5′S)-8,5′-Cyclo-2′-deoxyadenosines as Potential Biomarkers in Human Urine for Atherosclerosis

被引：36

作者：

Jaruga, Pawel ^{[1
]}

Rozalski, Rafal ^{[2
]}

Jawien, Arkadiusz ^{[3
]}

Migdaski, Arkadiusz ^{[3
]}

Olinski, Ryszard ^{[2
]}

Dizdaroglu, Miral ^{[1
]}

机构：

[1] NIST, Biochem Sci Div, Gaithersburg, MD 20899 USA

[2] Nicholas Copernicus Univ, Dept Clin Biochem, Bydgoszcz, Poland

[3] Nicholas Copernicus Univ, Dept Surg, Bydgoszcz, Poland

来源：

BIOCHEMISTRY | 2012年 / 51卷 / 09期

关键词：

EXCISION-REPAIR PATHWAY; OXIDATIVE STRESS; RISK-FACTOR; HUMAN-CELLS; MECHANISMS; DISEASE; 8-OXO-7,8-DIHYDRO-2'-DEOXYGUANOSINE; 8-OXO-7,8-DIHYDROGUANINE; MORTALITY; LESION;

D O I：

10.1021/bi201912c

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We hypothesized that DNA damage products (5'R)-8,5'-cyclo-2'-deoxyadenosine (R-cdA) and (5'S)-8,5'-cyclo-2'-deoxyadenosine (S-cdA) may be well-suited biomarkers of risk and diagnosis for atherosclerosis. We tested this hypothesis by measuring the levels of R-cdA and S-cdA and another product, 8-hydroxy-2'-deoxyguanosine (8-OH-dG), in urine of atherosclerosis patients and healthy individuals using liquid chromatography tandem mass spectrometry with isotope dilution. We showed the presence of these products at significantly greater concentrations in urine of atherosclerosis patients than in that of healthy individuals. Our data suggest that R-cdA and S-cdA. cart be accurately and reproducibly measured in human urine as potential biomarkers of risk and diagnosis for atherosclerosis.

引用

页码：1822 / 1824

页数：3

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