Genotoxicity of lapachol evaluated by wing spot test of Drosophila melanogaster

被引：6
|
作者：
Ferreira Costa, Wender [1 ]
Braga de Oliveira, Alaide [2 ]
Cesar Nepomuceno, Julio [1 ,3 ]
机构：
[1] Univ Fed Uberlandia, Inst Genet & Bioquim, BR-38400902 Uberlandia, MG, Brazil
[2] Univ Fed Minas Gerais, Dept Prod Farmaceut, Fac Farm, Belo Horizonte, MG, Brazil
[3] Ctr Univ Patos de Minas, Lab Citogenet & Mutagenese, Patos De Minas, MG, Brazil
来源：
GENETICS AND MOLECULAR BIOLOGY | 2010年 / 33卷 / 03期
关键词：
Drosophila melanogaster; SMART; lapachol; doxorubicin; cytochrome P450; SOMATIC MUTATION; RECOMBINATION TEST; DOXORUBICIN; CELLS; MECHANISMS; BIOACTIVATION; DERIVATIVES; ACID;
D O I：
10.1590/S1415-47572010005000070
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
This study investigated the genotoxicity of Lapachol (LAP) evaluated by wing spot test of Drosophila melanogaster in the descendants from standard (ST) and high bioactivation (HB) crosses. This assay detects the loss of heterozygosity of marker genes expressed phenotypically on the fly's wings. Drosophila has extensive genetic homology to mammals, which makes it a suitable model organism for genotoxic investigations. Three-day-old larvae from ST crosses (females flr(3)/TM3, Bd(s) x malesmwh/mwh), with basal levels of the cytochrome P450 and larvae of high metabolic bioactivity capacity (HB cross) (females ORR; flr(3)/TM3, Bd(s) x males mwh/mwh), were used. The results showed that LAP is a promutagen, exhibiting genotoxic activity in larvae from the HB cross. In other words, an increase in the frequency of spots is exclusive of individuals with a high level of the cytochrome P450. The results also indicate that recombinogenicity is the main genotoxic event induced by LAP.
引用
收藏
页码：558 / 563
页数：6
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