Biodesulfurization of Dibenzothiophene and Its Alkylated Derivatives in a Two-Phase Bubble Column Bioreactor by Resting Cells of Rhodococcus erythropolis IGTS8

被引：6

作者：

Prasoulas, George ^{[1
]}

Dimos, Konstantinos ^{[1
]}

Glekas, Panayiotis ^{[2
]}

Kalantzi, Styliani ^{[1
]}

Sarris, Stamatis ^{[3
]}

Templis, Chrysovalantis ^{[3
]}

Vavitsas, Konstantinos ^{[2
]}

Hatzinikolaou, Dimitris G. ^{[2
]}

Papayannakos, Nikolaos ^{[3
]}

Kekos, Dimitris ^{[1
]}

Mamma, Diomi ^{[1
]}

机构：

[1] Natl Tech Univ Athens, Sch Chem Engn, Biotechnol Lab, 9 Iroon Polytech Str,Zografou Campus, Athens 15780, Greece

[2] Natl & Kapodistrian Univ Athens, Dept Biol, Enzyme & Microbial Biotechnol Unit, Athens 15784, Greece

[3] Natl Tech Univ Athens, Sch Chem Engn, Chem Proc Engn Lab, 9 Iroon Polytech Str,Zografou Campus, Athens 15780, Greece

来源：

PROCESSES | 2021年 / 9卷 / 11期

关键词：

biodesulfurization kinetics; Rhodococcus erythropolis IGTS8; resting cells; dibenzothiophene; alkylated dibenzothiophene; bubble column bioreactor; OXYGEN-TRANSFER; DEEP DESULFURIZATION; KINETIC-ANALYSIS; MASS-TRANSFER; STIRRED-TANK; GROWTH-RATE; GORDONIA SP; CRUDE-OIL; SCALE-UP; BIOCATALYST;

D O I：

10.3390/pr9112064

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

Biodesulfurization (BDS) is considered a complementary technology to the traditional hydrodesulfurization treatment for the removal of recalcitrant sulfur compounds from petroleum products. BDS was investigated in a bubble column bioreactor using two-phase media. The effects of various process parameters, such as biocatalyst age and concentration, organic fraction percentage (OFP), and type of sulfur compound-namely, dibenzothiophene (DBT), 4-methyldibenzothiophene (4-MDBT), 4,6-dimethyldibenzothiophene (4,6-DMDBT), and 4,6-diethyldibenzothiophene (4,6-DEDBT)-were evaluated, using resting cells of Rhodococcus erythropolis IGTS8. Cells derived from the beginning of the exponential growth phase of the bacterium exhibited the highest biodesulfurization efficiency and rate. The biocatalyst performed better in an OFP of 50% v/v. The extent of DBT desulfurization was dependent on cell concentration, with the desulfurization rate reaching its maximum at intermediate cell concentrations. A new semi-empirical model for the biphasic BDS was developed, based on the overall Michaelis-Menten kinetics and taking into consideration the deactivation of the biocatalyst over time, as well as the underlying mass transfer phenomena. The model fitted experimental data on DBT consumption and 2-hydroxibyphenyl (2-HBP) accumulation in the organic phase for various initial DBT concentrations and different organosulfur compounds. For constant OFP and biocatalyst concentration, the most important parameter that affects BDS efficiency seems to be biocatalyst deactivation, while the phenomenon is controlled by the affinities of biodesulfurizing enzymes for the different organosulfur compounds. Thus, desulfurization efficiency decreased with increasing initial DBT concentration, and in inverse proportion to increases in the carbon number of alkyl substituent groups.

引用

页数：19