In vivo simultaneous multispectral fluorescence imaging with spectral multiplexed volume holographic imaging system

被引：6
|
作者：
Lv, Yanlu [1 ]
Zhang, Jiulou [1 ]
Zhang, Dong [1 ]
Cai, Wenjuan [1 ]
Chen, Nanguang [2 ]
Luo, Jianwen [1 ,3 ]
机构：
[1] Tsinghua Univ, Dept Biomed Engn, Beijing 100084, Peoples R China
[2] Natl Univ Singapore, Dept Biomed Engn, Singapore 117576, Singapore
[3] Tsinghua Univ, Ctr Biomed Imaging Res, Beijing 100084, Peoples R China
来源：
JOURNAL OF BIOMEDICAL OPTICS | 2016年 / 21卷 / 06期
基金：
中国国家自然科学基金;
关键词：
simultaneous multispectral fluorescence imaging; volume holographic imaging system; multiplexed volume holographic grating; multiple fluorophores; MICROSCOPY;
D O I：
10.1117/1.JBO.21.6.060502
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A simultaneous multispectral fluorescence imaging system incorporating multiplexed volume holographic grating (VHG) is developed to acquire multispectral images of an object in one shot. With the multiplexed VHG, the imaging system can provide the distribution and spectral characteristics of multiple fluorophores in the scene. The implementation and performance of the simultaneous multispectral imaging system are presented. Further, the system's capability in simultaneously obtaining multispectral fluorescence measurements is demonstrated with in vivo experiments on a mouse. The demonstrated imaging system has the potential to obtain multispectral images fluorescence simultaneously. (C) 2016 Society of Photo-Optical Instrumentation Engineers (SPIE)
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页数：4
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