An effective validated method for HPTLC-fingerprinting of alkaloids and glycosides from multiple plant parts of three Terminalia spp.

被引：1

作者：

Hakim, Masuma ^{[1
]}

Rathod, Dipika ^{[1
]}

Trivedi, Devanshi A. ^{[1
]}

Panigrahi, Jitendriya ^{[2
]}

Gantait, Saikat ^{[3
,4
]}

Patel, Illa C. ^{[1
]}

机构：

[1] Hemchandracharya North Gujarat Univ, Dept Life Sci, Patan 384265, Gujarat, India

[2] Shri AN Patel Postgrad Inst Sci & Res, Dept Biotechnol, Anand 388001, Gujarat, India

[3] Bidhan Chandra Krishi Viswavidyalaya, Directorate Res, All India Coordinated Res Project Groundnut, Nadia 741235, W Bengal, India

[4] Bidhan Chandra Krishi Viswavidyalaya, Fac Agr, Dept Genet & Plant Breeding, Nadia 741252, W Bengal, India

来源：

ISRAEL JOURNAL OF PLANT SCIENCES | 2018年 / 65卷 / 1-2期

关键词：

Alkaloids; Chromatography; Fingerprint; Glycosides; HPTLC; Terminalia; TLC; L; QUANTIFICATION; FORMULATIONS; BELLERICA; TANNINS; FRUIT; ACID;

D O I：

10.1163/22238980-06401002

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Our study developed a HPTLC fingerprint profile of alkaloids and glycosides obtained from the methanol extracts of four different plant parts of Terminalia arjuna, T. bellerica and T. chebula, trees with cardio-protective values. The multiple qualitative phytochemical analyses of water, acetone, petroleum ether and methanol extracts from all the plant parts of Terminalia spp. detected the presence of alkaloids and glycosides, wherein the methanol extracts exhibited the presence of maximum alkaloids and glycosides. The chromatographic analysis of methanol extracts was carried out on silica gel 60F254 HPTLC aluminium sheets with CAMAG Linomat 5 applicator. The plates were developed using ethyl acetate: toluene: formic acid (10:10:1; v/v/v) mobile phase. Alkaloids and glycosides were detected at 254 nm, 366 nm and 540 nm (after derivatization). These developed fingerprints would eventually be of great benefit in identifying or differentiating the alkaloids and glycosides in the form of marker compounds in the three Terminalia spp. mentioned.

引用

页码：109 / 117

页数：9

共 1 条

[1] A reversed-phase HPLC-UV method developed and validated for simultaneous quantification of six alkaloids from Nicotiana spp.
Moghbel, Nahid
Ryu, BoMi
Steadman, Kathryn J.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2015, 997 : 142 - 145

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