Isolation, purification and characterization of naturally derived Crocetin beta-D-glucosyl ester from Crocus sativus L. against breast cancer and its binding chemistry with ER-alpha/HDAC2

被引：37

作者：

Mir, Mudasir A. ^{[1
]}

Ganai, Shabir Ahmad ^{[2
]}

Mansoor, Sheikh ^{[3
]}

Jan, Sumira ^{[4
]}

Mani, P. ^{[5
]}

Masoodi, Khalid Z. ^{[1
]}

Amin, Henna ^{[6
]}

Rehman, Muneeb U. ^{[7
]}

Ahmad, Parvaiz ^{[8
]}

机构：

[1] Sher E Kashmir Univ Agr Sci & Technol Kashmir, Div Biotechnol, Srinagar 190025, Jammu & Kashmir, India

[2] SKUAST Kashmir, Fac Agr, Div Basic Sci & Humanities, Sopore 193201, Jammu & Kashmir, India

[3] Sher E Kashmir Univ Agr Sci & Technol, Div Biochem, Jammu 180009, J&K, India

[4] Sher E Kashmir Univ Agr Sci & Technol, Div Basic Sci & Humanities, Srinagar 190025, Jammu & Kashmir, India

[5] Annai Coll Arts & Sci, Dept Biotechnol, Kumbakonam 612503, Tamil Nadu, India

[6] Univ Kashmir, Fac Appl Sci & Technol, Dept Pharmaceut Sci, Srinagar 190006, J&K, India

[7] King Saudi Univ, Coll Pharm, Dept Clin Pharm, POB 2460, Riyadh 11451, Saudi Arabia

[8] King Saudi Univ, Coll Sci, Bot & Microbiol Dept, POB 2460, Riyadh 11451, Saudi Arabia

来源：

SAUDI JOURNAL OF BIOLOGICAL SCIENCES | 2020年 / 27卷 / 03期

关键词：

Saffron; Floral biowastes; Antioxidant; Crocetin beta-D-glucosyl ester; Breast cancer; Molecular docking; SAFFRON CAROTENOIDS; IN-VITRO; PROTEIN; DOCKING; CHEMOPREVENTION; INHIBITION; PREDICTION; THERAPY; FLOWERS; GLIDE;

D O I：

10.1016/j.sjbs.2020.01.018

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Saffron plant (Crocus sativus L.) is being used as a source of saffron spice and medicine to cure or prevent different types of diseases including cancers. We report the isolation, characterization of bioactive small molecule ([crocetin (beta-D-glucosyl) ester] from the leaf biowastes of saffron plant of Kashmir, India. MTTC assay and Bio-autography aided approach were used to assess anti-oxidant activity and anti-cancer properties of crocin (s) against DPPH free radical and breast cancer cell line respectively. Crocetin beta-D-glucosyl ester restrained proliferation of human breast adeno-carcinoma cell model (MCF-7) without significantly affecting normal cell line (L-6). Further studies involving molecular mechanics generalized born surface area and molecular docking showed that crocetin beta-D-glucosyl ester exhibits strong affinity for estrogen receptor alpha and histone deacetylase 2 (crucial receptors involved in breast cancer signalling) as evidenced by the negative docking score and binding free energy (BFE) values. Therefore, crocetin beta-D-glucosyl ester from Crocus sativus biowastes showed antiproliferative effect possibly by inhibiting estrogen receptor alpha and HDAC2 mediated signalling cascade. (C) 2020 The Author(s). Published by Elsevier B.V. on behalf of King Saud University.

引用

页码：975 / 984

页数：10