The software system design model based on digital PCR fluorescence detector

被引:1
|
作者
Li, Xinxin [1 ,2 ]
Wu, Shaofei [1 ,2 ]
Wang, Mingqing [3 ]
Zou, Yuntao [4 ]
机构
[1] Wuhan Inst Technol, Hubei Prov Key Lab Intelligent Robots, Wuhan, Hubei, Peoples R China
[2] Wuhan Inst Technol, Sch Comp Sci & Engn, Wuhan, Hubei, Peoples R China
[3] Wuhan CarCloud Technol Pte Ltd, Wuhan, Hubei, Peoples R China
[4] Wuhan Winphone Technol Co Ltd, Wuhan, Hubei, Peoples R China
关键词
Digital PCR; Fluorescence detector; Data analysis; Life sciences and computer; Instrument control;
D O I
10.1007/s10586-018-1931-7
中图分类号
TP [自动化技术、计算机技术];
学科分类号
0812 ;
摘要
Digital PCR generally includes two parts. That is, the PCR amplification and fluorescence signal analysis. In the PCR amplification stage. Using digital PCR to dilute samples onto a single molecule level, the average to dozens to tens of thousands of units in chemical reaction, make each reaction unit of target molecules is 0 or 1, makes the target molecules positive signal detection. One of them will use the upper software control laboratory PCR instrument operation, instrument of the data acquisition, as well as to the scientific analysis and processing the data collected, is advantageous for the quantitative analysis of the gene and detection. The software to control one PCR instrument operation and work. In addition this instrument will collect data uploaded to PC through USB interface, through analysis and deal with the PC software. Finally experimental conclusions.
引用
收藏
页码:S8623 / S8627
页数:5
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