Photoelectrochemical immunodiagnosis of canine leishmaniasis using cadmium-sulfide-sensitized zinc oxide modified with synthetic peptides

被引：9
|
作者：
Neto, Sakae Yotsumoto [1 ]
Soares da Silva, Fernanda Gabrielle [1 ]
Pires Souto, Denio Emanuel [2 ,3 ]
Faria, Angelica Rosa [4 ]
de Andrade, Helida Monteiro [4 ]
Silva Luz, Rita de Cassia [1 ,2 ]
Kubota, Lauro Tatsuo [2 ,3 ]
Damos, Flavio Santos [1 ,2 ]
机构：
[1] Fed Univ Maranhao UFMA, Dept Chem, BR-65080805 Sao Luis, MA, Brazil
[2] Natl Inst Sci & Technol Bioanalyt, BR-13083970 Campinas, SP, Brazil
[3] State Univ Campinas UNICAMP, Inst Chem, BR-13083970 Campinas, SP, Brazil
[4] Fed Univ Minas Gerais UFMG, Inst Biol Sci, BR-3127991 Belo Horizonte, MG, Brazil
来源：
ELECTROCHEMISTRY COMMUNICATIONS | 2017年 / 82卷
关键词：
Photoelectrochemical immunosensor; Visceral leishmaniasis; Visible LED light; Cadmium sulfide; Zinc oxide; REGENERATION KINETICS; INFANTUM-ANTIBODIES; IMMUNOSENSOR; PROTEIN; BIOSENSORS; DIAGNOSIS; ZNO; SPR;
D O I：
10.1016/j.elecom.2017.07.027
中图分类号：
O646 [电化学、电解、磁化学];
学科分类号：
081704 ;
摘要：
A photoelectrochemical immunosensor for the detection of anti-Leishmania infantum antibodies based on zinc oxide and cadmium sulfide films electrodeposited on an ITO-coated glass slide (CdS/ZnO/ITO) has been proposed. The effects of light/dark conditions and the kinetics of CdS sensitizer regeneration were evaluated by scanning electrochemical microscopy in feedback mode. The platform was modified using two different peptides (PEP 13 and PEP 16) from two different proteins of high specificity and selectivity toward recognition of L. infantum antibodies, producing Peps/CdS/ZnO/ITO. This photoelectrochemical immunosensor provides a cheap and promising method of discriminating between positive and negative canine serum samples.
引用
收藏
页码：75 / 79
页数：5
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