Platelet-derived growth factor-BB (PDGF-BB) improves follicular survival, oocyte and follicular diameters, in a dose-dependent manner, after the in vitro culture of goat preantral follicles enclosed in ovarian tissue fragments

被引：2

作者：

da Silva, Renato Felix ^{[1
]}

Brito, Ivina Rocha ^{[1
]}

de Lima, Laritza Ferreira ^{[1
]}

Nascimento de Aguiar, Francisco Leo ^{[1
]}

Rodrigues, Giovanna Quintino ^{[1
]}

Castro do Nascimento, Ivila Lorrine ^{[1
]}

Pedrosa Rocha, Rebeca Magalhaes ^{[1
]}

Santos Cibin, Francielli Weber ^{[2
]}

Wheeler, Matthew Bryan ^{[3
]}

Campello, Claudio Cabral ^{[1
]}

Ribeiro Rodrigues, Ana Paula ^{[1
]}

de Figueiredo, Jose Ricardo ^{[1
]}

机构：

[1] Univ Estadual Ceara, Fac Vet, Lab Manipulat Oocytes & Preantral Follicles, Itaperi Campus, Fortaleza, Ceara, Brazil

[2] Fed Univ Pampa, Uruguaiana, RS, Brazil

[3] Univ Illinois, Dept Anim Sci, 328 Mumford Hall, Urbana, IL 61801 USA

来源：

ANIMAL REPRODUCTION | 2017年 / 14卷 / 04期

关键词：

goat; in vitro culture; PDGF-BB; preantral follicles; PRIMORDIAL FOLLICLES; GENE-EXPRESSION; RECEPTORS; CELLS; ACTIVATION; MATURATION; VIABILITY; BOVINE; DIFFERENTIATION; INFLAMMATION;

D O I：

10.21451/1984-3143-AR935

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

The aims of this study were to investigate the effects of different concentrations of Platelet-derived growth factor-BB (PDGF-BB) on the survival, activation, levels of ROS, and growth of goat preantral follicles enclosed in ovarian tissue. For this, ovarian fragments were cultured for 7 days in Alpha Minimum Essential Medium (alpha-MEM+) with or without PDGF-BB (0, 25, 50 and 100 ng/ml). The results showed that both the 25 ng/ml PDGF and the 50 ng/ml PDGF treatments maintained the percentage of morphologically normal follicles from day 1 to day 7. In addition, the 25 ng/ml PDGF treatment showed a significantly higher percentage of morphologically normal follicles when compared to the other treatments. At day 7, greater (P < 0.05) follicular and oocyte diameters were observed in the 25 ng/ml PDGF and the 50 ng/ml PDGF treatments when compared to the cultured control treatment. On day 7 of culture, all the treatments tested had a significant increase in the percentage of developing follicles when compared to the non-cultured control. However, the percentage of follicle activation, as well as ROS production, were similar (P < 0.05) among the treatments, irrespective of culture time. In conclusion, PDGF-BB improved, in a concentration-dependent manner, follicular survival as well as oocyte and follicular diameter after in vitro culture of goat preantral follicle-enclosed in ovarian tissue fragments.

引用

页码：1095 / 1102

页数：8