Thin-layer cyclic voltammetric and scanning electrochemical microscopic study of antioxidant activity of ascorbic acid at liquid/liquid interface

An electron transfer reaction between ascorbic acid (H(2)A) in an aqueous solution and oxidizing agent in an organic solution immiscible with water has been studied by thin-layer cyclic voltammetry (TLCV) for charge transfer at the interface between two immiscible electrolyte solutions (ITIES). As an antioxidant, H(2)A provide electrons through the aqueous/organic interface to reduce Fc(+) and the procedure has been proved to be a one electron process again. In this work, the first combination of TLCV and scanning electrochemical microscopy (SECM) was achieved and showed a reasonable agreement between the results from the two different approaches. Otherwise, lower concentration ratios Kr of aqueous to organic reactants was adopted, which is given as evidence to the proposed procedure of Barker.