Improved biocatalysts by directed evolution and rational protein design

被引：339
作者：
Bornscheuer, UT
Pohl, M
机构：
[1] Ernst Moritz Arndt Univ Greifswald, Inst Chem & Biochem, Dept Tech Chem & Biotechnol, D-17487 Greifswald, Germany
[2] MPB Cologne GmbH, D-51063 Cologne, Germany
来源：
CURRENT OPINION IN CHEMICAL BIOLOGY | 2001年 / 5卷 / 02期
关键词：
D O I：
10.1016/S1367-5931(00)00182-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The efficient application of biocatalysts requires the; availability of suitable: enzymes with high activity and stability under process conditions, desired substrate selectivity and high enantioselectivity. However, wild-type enzymes: often need to be optimized La fulfill these requirements. Two: rather contradictory tools can be used on a, molecular level to create tailor-made bio-catalysts: directed evolution and rational protein design.
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收藏
页码：137 / 143
页数：7
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