Elasticity of cardiac cells on the polymer substrates with different stiffness: an atomic force microscopy study

被引：34

作者：

Shi, Xiaoli ^{[1
]}

Qin, Lei ^{[2
]}

Zhang, Xuejie ^{[1
]}

He, Kangmin ^{[1
,3
]}

Xiong, Chunyang ^{[3
]}

Fang, Jing ^{[3
]}

Fang, Xiaohong ^{[1
]}

Zhang, Youyi ^{[2
]}

机构：

[1] Chinese Acad Sci, Beijing Natl Lab Mol Sci, Key Lab Mol Nanostruct & Nanotechnol, Inst Chem, Beijing 100190, Peoples R China

[2] Peking Univ, Inst Vasc Med, Hosp 3, Minist Educ Key Lab Mol, Beijing 100191, Peoples R China

[3] Peking Univ, Acad Adv Interdisciplinary Studies, Beijing 100191, Peoples R China

来源：

PHYSICAL CHEMISTRY CHEMICAL PHYSICS | 2011年 / 13卷 / 16期

基金：

中国国家自然科学基金;

关键词：

LIVING CELLS; CYTOSKELETAL STRUCTURE; FIBROBLASTS; HEART; LOCOMOTION; MECHANICS; RIGIDITY; ADHESION;

D O I：

10.1039/c1cp20154a

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Influences of substrate stiffness on mechanical properties of cardiac myocytes and fibroblasts were investigated by cell elasticity measurement with atomic force microscopy. The cells were cultured on collagen-coated polyacrylamide substrates with gradient rigidity. While cardiac myocytes showed no evident change in cell elasticity on different substrates, cardiac fibroblasts displayed the non-monotonic dependence on substrate stiffness with a maximum elastic modulus. Moreover, the elasticity change of cardiac fibroblasts with substrates stiffness was found to be regulated by actin filaments. Study of the effect of substrate stiffness on cell elasticity for different cardiac cells provides new information for the better understanding of cardiac physiology and pathology.

引用

页码：7540 / 7545

页数：6

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