Hymenobacter jejuensis sp. nov., a UV radiation-tolerant bacterium isolated from Jeju Island

A novel Gram-stain negative, aerobic, rod-shaped, non-motile and pink-coloured bacterium, designated strain 17J68-5(T), was isolated from soil in Jeju Island, Korea. The strain was found to grow at 18-37 degrees C (optimum 25 degrees C) in R2A medium at pH (6.0 to 7.5; optimum 6.5) in the presence of 0% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 17J68-5(T) forms a distinct lineage within the family Hymenobacteraceae and is closely related to Hymenobacter daecheongensis DSM 21074(T) (94.9% 16S rRNA gene sequence similarity), Hymenobacter rutilus K2-33028(T) (94.6%) and Hymenobacter tibetensis XTM003(T) (94.3%). The draft genome sequence of strain 17J68-5(T)is 5.1 Mb size. The calculated average nucleotide identity and the digital DNA-DNA hybridization between strain 17J68-5(T) and closely related type strains were 81.3 to 84.1 % and 25.5 to 28.1%. The major cellular fatty acids (>= 10%) of the strain 17J68-5(T) were identified as summed feature 3 (C-16:1 omega 6c/C-16:1 omega 7c; 21.2%), iso-C-15:0 (19.1%), summed feature 4 (C-17:1 iso I/C-17:1 anteiso B; 17.9%) and C-16:1 omega 5c (13.1%). The predominant respiratory quinones were found to be menaquinone 7 and 6 (MK-7 and MK-6). The major polar lipid was found to be phosphatidylethanolamine. The genomic DNA G + C content based on the whole genome sequence is 59.6 mol %. The phenotypic, chemotaxonomic and genotypic properties clearly indicated that isolate 17J68-5(T) represents a novel species within the genus Hymenobacter, for which the name Hymenobacter jejuensis sp. nov. is proposed. The type strain of Hymenobacter jejuensis is 17J68-5(T) (= KCTC 62224(T) = JCM 33182(T)).