Expression profiling of single cells using 3 prime end amplification (TPEA) PCR

被引：68
作者：
Dixon, AK
Richardson, PJ
Lee, K
Carter, NP
Freeman, TC
机构：
[1] Sanger Ctr, Cambridge CB10 1SA, England
[2] Univ Cambridge, Dept Pharmacol, Cambridge CB2 1QJ, England
[3] Parke Davis Neurosci Res Ctr, Cambridge CB2 2QB, England
来源：
NUCLEIC ACIDS RESEARCH | 1998年 / 26卷 / 19期
基金：
英国惠康基金;
关键词：
D O I：
10.1093/nar/26.19.4426
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The ability to relate the physiological status of individual cells to the complement of genes they express is limited by current methodological approaches for performing these analyses. We report here the development of a robust and reproducible method for amplifying 3' sequences of mRNA derived from single cells and demonstrate that the amplified cDNA, derived from individual human lymphoblastoma cells, can be used for the expression profiling of up to 40 different genes per cell. In addition, we show that 3 prime end amplification (TPEA) PCR can be used to enable the detection of both high and low abundance mRNA species in samples harvested from live neurons in rat brain slices. This procedure will facilitate the study of complex tissue function at the cellular level.
引用
收藏
页码：4426 / 4431
页数：6
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