Direct electrochemistry of horse spleen ferritin on modified gold electrode

The direct electron transfer of ferritin modified covalently on gold electrode was investigated. Cyclic voltammetry was a principal method used to investigate the reactivity of the adsorbed layer. Moreover, the process of modified electrode was characterized by surface plasma resonance (SPR) and the surface coverage was obtained. On this modified electrode, ferritin exhibited reasonably well-defined current-potential curves. However, no stable electrochemical signal was obtained after consecutive cycle; this might be resulted from the denatureation of ferritin after multicycle scan. In addition, the mechanism of electrochemically regulated iron uptake and release for ferritin was proposed.