Monitoring adsorption in situ and kinetic studies of low-density lipoprotein onto cholesterol modified dextran using quartz crystal microbalance

The use of quartz crystal microbalance(QCM) for monitoring in situ the adsorption of low-density lipoprotein(LDL) onto cholesterol modified dextran(CMD) was described. The kinetic studies were made. The experimental results show that kinetic curves are fitted for that of Langmuir adsorption equation quite well, when LDL mass concentration (ng/mu L) are 9.9, 12.38, 14.14, respectively, and the rate constants for adsorption(mol center dot L-1 center dot s(-1)) and adsorption capacity (ng/cm(2)) are estimated to be 0.047 7, 0.053 6, 0.062 8 and 107.6, 139.6, 167.9, respectively. The maximal LDL adsorption capacity attains 284.8 ng/cm(2) and the adsorptive efficiency was calculated as 72.91%.