Two proline-rich peptides from pig (Sus scrofa) salivary glands generated by pre-secretory pathway underlying the action of a proteinase cleaving Pro-Ala bonds

被引：12

作者：

Patamia, A

Messana, I

Petruzzelli, R

Vitali, A

Inzitari, R

Cabras, T

Fanali, C

Scarano, E

Contucci, A

Galtieri, A

Castagnola, M

机构：

[1] Catholic Univ, Fac Med, Ist Biochim & Biochim Clin, Ist Chim Riconoscimento Mol,CNR, I-00168 Rome, Italy

[2] Univ Cagliari, Dipartimento Sci Applicate & Bosistemi, Cagliari, Italy

[3] Univ G DAnnunzio, Dipartimento Sci Biomed, Chieti, Italy

[4] Catholic Univ, Fac Med, Ist Otorinolaringoiatria, Rome, Italy

[5] Univ Messina, Dipartimento Chim Organ & Biol, Messina, Italy

来源：

PEPTIDES | 2005年 / 26卷 / 09期

关键词：

proline-rich proteins; saliva; salivary glands; parotid; pig; Sus scrofa; prolyl-alanyl proteinase;

D O I：

10.1016/j.peptides.2005.02.021

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The primary structures of two salivary proline-rich peptides (PRP-SP-A, M 6156.0 amu and PRP-SP-B, M 1905.0 amu), from pig (Sus scrofa) were determined. The PRP-SP-B peptide, 21 residues long, overlaps with a sequence repeated 43 times in three deposited cDNAs coding for PRP proteins cloned from porcine parotid glands (Swiss-Prot codes: Q95JC9, Q95JD1, Q95JD0). PRP-SP-A peptide, 56 amino acid residues long, overlaps with the N-terminus repeats of Q95JC9 and Q95JD1 and it is phosphorylated at Ser 12 and 14. The two peptides were found both in whole saliva and in granules from pig parotid glands. The biosynthesis of the two peptides implies the action of a proteinase responsible for Pro down arrow Ala cleavage in the pre-secretory process. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：1550 / 1559

页数：10