Purpose: A retrospective review of preoperative three-dimensional (3D) CT and the operative findings during laparoscopic donor nephrectomy. Patients and Methods: Fifty-four consecutive patients underwent laparoscopic donor nephrectomy. Of these patier ts, 51 had preoperative 3D reconstructed CT scans. Each radiologic report was compared with the operative report. Results: The 3D CT correctly identified the arteries in 98% of the patients and the veins in 96%. Conclusions: Preoperative CT angiography can accurately identify the renal vasculature.