Antibiotic saving effect of combination therapy through synergistic interactions between well-characterized chito-oligosaccharides and commercial antifungals against medically relevant yeasts

被引：13

作者：

Ganan, Monica ^{[1
,2
]}

Lorentzen, Silje B. ^{[1
]}

Aam, Berit B. ^{[1
]}

Eijsink, Vincent G. H. ^{[1
]}

Gaustad, Peter ^{[2
]}

Sorlie, Morten ^{[1
]}

机构：

[1] Norwegian Univ Life Sci, Dept Chem Biotechnol & Food Sci, As, Norway

[2] Univ Oslo, Inst Clin Med, Dept Microbiol, Oslo, Norway

来源：

PLOS ONE | 2019年 / 14卷 / 12期

关键词：

CANDIDA-ALBICANS; ASPERGILLUS-FUMIGATUS; AMPHOTERICIN-B; EFFECT PAFE; TIME-KILL; IN-VITRO; FLUCONAZOLE; INFECTIONS; RESISTANCE; EXPOSURE;

D O I：

10.1371/journal.pone.0227098

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Combination therapies can be a help to overcome resistance to current antifungals in humans. The combined activity of commercial antifungals and soluble and well-defined low molecular weight chitosan with average degrees of polymerization (DPn) of 17-62 (abbreviated C17-C62) and fraction of acetylation (F-A) of 0.15 against medically relevant yeast strains was studied. The minimal inhibitory concentration (MIC) of C32 varied greatly among strains, ranging from > 5000 mu g mL(-1) (Candida albicans and C. glabrata) to < 4.9 (C. tropicalis). A synergistic effect was observed between C32 and the different antifungals tested for most of the strains. Testing of several CHOS preparations indicated that the highest synergistic effects are obtained for fractions with a DPn in the 30-50 range. Pre-exposure to C32 enhanced the antifungal effect of fluconazole and amphotericin B. A concentration-dependent post-antifungal effect conserved even 24 h after C32 removal was observed. The combination of C32 and commercial antifungals together or as part of a sequential therapy opens new therapeutic perspectives for treating yeast infections in humans.

引用

页数：13