Intra-abdominal adhesion formation induces anti-oxidative injury, enhances cell proliferation, and prevents complement-mediated lysis

被引：7

作者：

Yu, Sung-Liang ^{[2
,3
]}

Singh, Sher ^{[4
]}

Chen, Huei-Wen ^{[5
]}

Chen, Hsuan-Yu ^{[6
]}

Chen, Jeremy J. W. ^{[7
]}

Chen, Wen-Jone ^{[1
]}

Chen, Han-Shiang ^{[8
]}

Chen, Shyr-Chyr ^{[1
]}

机构：

[1] Natl Taiwan Univ Hosp, Dept Emergency Med, Taipei 10016, Taiwan

[2] Natl Taiwan Univ, Coll Med, Dept Clin Lab Sci & Med Biotechnol, Taipei 10764, Taiwan

[3] Natl Taiwan Univ Hosp, Dept Med Res, Taipei 10016, Taiwan

[4] Natl Taiwan Normal Univ, Dept Life Sci, Taipei, Taiwan

[5] Natl Yang Ming Univ, Inst Pharmacol, Taipei 112, Taiwan

[6] Acad Sinica, Inst Stat Sci, Taipei 11529, Taiwan

[7] Natl Chung Hsing Univ, Inst Biomed Sci & Mol Biol, Taichung 40227, Taiwan

[8] Hualien Tzu Chi Med Ctr, Dept Surg, Hualien, Taiwan

来源：

WOUND REPAIR AND REGENERATION | 2008年 / 16卷 / 03期

基金：

中国国家自然科学基金;

关键词：

D O I：

10.1111/j.1524-475X.2008.00378.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Whether the alteration of gene expression is accompanied with intra-abdominal adhesion formation is unclear. The aim of this study was to analyze the dynamic gene expression patterns in an animal model of intra-abdominal adhesion formation. The mRNA was extracted from the jejunums of sham control mice and jejunum-abrading mice at 1, 3, 7, and 14 days postsurgery. The mouse cDNA microarray was used to monitor the dynamic changes of the tested genes and up-regulated and down-regulated genes were calculated. Quantitative real-time RT-PCR, and immunohistochemistry staining were used to confirm the accuracy of microarray results at RNA and protein levels. The top 100 genes with the greatest change across all studied mice groups were identified and 93 of them were correct after sequencing verification. Of the 93 genes, 74 genes were up-regulated and 19 were down-regulated following jejunal abrasion. Gene expressions of complement-mediated lysis, anti-oxidative response, and cell proliferation were significantly induced during adhesion formation. Intra-abdominal adhesion induces several genes to eliminate overfilled complement-mediated lysis, prevent oxidative injuries, and enhance cell proliferation. These findings may provide insights into the pathogenesis of intra-abdominal adhesion formation and might also help to identify some new target genes for specific diagnostic tools and novel therapeutic strategies.

引用

页码：388 / 398

页数：11