Enhancement of stress tolerance in Saccharomyces cerevisiae by overexpression of ubiquitin ligase Rsp5 and ubiquitin-conjugating enzymes

被引：36
|
作者：
Hiraishi, Hiroyuki [1 ]
Mochizuki, Mai [1 ]
Takagi, Hiroshi [1 ]
机构：
[1] Fukui Prefectural Univ, Dept Biosci, Fukui 9101195, Japan
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2006年 / 70卷 / 11期
关键词：
Rsp5 ubiquitin ligase; ubiquitin-conjugating enzyme; stress tolerance; Saccharomyces cerevisiae;
D O I：
10.1271/bbb.60250
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Yeast Saccharomyces cerevisiae cells overexpressing essential ubiquitin ligase Rsp5 or ubiquitin-conjugating enzymes (Ubc1-Ubc13) showed tolerance to various stresses. Co-overexpression of Rsp5 and Ubc1, Ubc2, Ubc3, Ubc5, Ubc6, Ubc9, Ubc10, Ubc11, Ubc12, or Ubc13 further enhanced stress tolerance. These results suggest that overexpression of ubiquitin-related enzymes might be a useful method for breeding novel stress-resistant strains.
引用
收藏
页码：2762 / 2765
页数：4
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