Study of Blood Serum in Rats with Transplanted Cholangiocarcinoma Using Raman Spectroscopy

被引：7
|
作者：
Mankova, A. A. [1 ]
Cherkasova, O. P. [2 ,3 ]
Lazareva, E. N. [4 ,5 ]
Bucharskaya, A. B. [6 ]
Dyachenko, P. A. [4 ,5 ]
Kistenev, Yu, V [4 ,7 ]
Vrazhnov, D. A. [4 ,8 ]
Skiba, V. E. [4 ]
Tuchin, V. V. [4 ,5 ,9 ]
Shkurinov, A. P. [1 ,3 ]
机构：
[1] Moscow MV Lomonosov State Univ, Moscow 119991, Russia
[2] Russian Acad Sci, Inst Laser Phys, Siberian Branch, Novosibirsk 630090, Russia
[3] RAS, Inst Laser & Informat Technol Problems, Fed Res Ctr Crystallog & Photon, Shatura 140700, Russia
[4] Natl Res Tomsk State Univ, Tomsk 634050, Russia
[5] Saratov Natl Res State Univ, Saratov 410012, Russia
[6] Saratov State Med Univ, Saratov 410012, Russia
[7] Siberian State Med Univ, Tomsk 634050, Russia
[8] Russian Acad Sci, Inst Strength Phys & Mat Sci, Siberian Branch, Tomsk 634055, Russia
[9] Russian Acad Sci, Inst Precis Mech & Control, Saratov 410028, Russia
来源：
OPTICS AND SPECTROSCOPY | 2020年 / 128卷 / 07期
基金：
俄罗斯基础研究基金会;
关键词：
Raman spectroscopy; blood serum; experimental liver cancer; principal component analysis; SECONDARY STRUCTURE; AMIDE-I; PROTEINS; DIAGNOSTICS; ALBUMIN; SAMPLES; TOOL;
D O I：
10.1134/S0030400X20070115
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
Serum samples of healthy rats and rats with inoculated tumors on days 14 and 28 after transplantation of cholangiocarcinoma cells were analyzed using Raman spectroscopy (RS). It was shown that the intensity of the amide 1 band differs in the serum spectra depending on the stage of tumor development and correlates with the protein concentration in the samples. In some animals, there is a significant increase in the blood glucose concentration, leading to glycation of proteins and a change in the shape of the amide 1 band. The appearance of a band at 1670 cm(-1)is most pronounced in the spectra of samples on the 28th day of the experiment and may be associated with an increase in the content of beta-structural elements in the protein conformation. Using the principal component analysis, we assessed the general differences in the Raman spectra of the blood serum in rats of control groups and rats on the 28th day after tumor transplantation.
引用
收藏
页码：964 / 971
页数：8
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