Quantification of urinary 18-hydroxycortisol using LC-MS/MS

被引：6
|
作者：
Jin, Shigeki [1 ]
Wada, Norio [2 ]
Takahashi, Yuji [1 ,3 ]
Hui, Shu-Ping [1 ]
Sakurai, Toshihiro [1 ]
Fuda, Hirotoshi [1 ]
Takeda, Seiji [1 ]
Fujikawa, Masato [3 ]
Yanagisawa, Katsuyuki [2 ]
Ikegawa, Shigeo [4 ]
Kurosawa, Takao [5 ]
Chiba, Hitoshi [1 ]
机构：
[1] Hokkaido Univ, Fac Hlth Sci, Sapporo, Hokkaido 0600812, Japan
[2] Sapporo City Gen Hosp, Dept Endocrinol & Diabet, Sapporo, Hokkaido, Japan
[3] Sapporo City Gen Hosp, Dept Clin Lab, Sapporo, Hokkaido, Japan
[4] Kinki Univ, Fac Pharmaceut Sci, Higashiosaka, Osaka 577, Japan
[5] Hlth Sci Univ Hokkaido, Fac Pharmaceut Sci, Hokkaido, Tobetsu, Japan
来源：
ANNALS OF CLINICAL BIOCHEMISTRY | 2013年 / 50卷 / 05期
基金：
日本学术振兴会;
关键词：
Steroid; mass spectrometry; aldosteronism; hypertension; PERFORMANCE LIQUID-CHROMATOGRAPHY; PRIMARY ALDOSTERONISM; SUBTYPE DIFFERENTIATION; ENZYME-IMMUNOASSAY; DIAGNOSIS; HYPERALDOSTERONISM; CORTICOSTEROIDS; 18-OXOCORTISOL; PREVALENCE;
D O I：
10.1177/0004563213476272
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
Background: Urinary 18-hydroxycortisol has been investigated as a marker of aldosterone-producing adenoma (APA). The aim of this study was to develop and validate a method for the measurement of 18-hydroxycortisol using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Methods: Urine was collected over a 24-hour period in patients with APA (n=11), idiopathic hyperaldosteronism (IHA, n=9), and essential hypertension (EH, n=6). 18-Hydroxycortisol was extracted in solid-phase, and measured by LC-MS/MS based on selected reaction monitoring. Results: The method allowed quantification of 18-hydroxycortisol with a lower quantification limit of 0.26nmol/L, intra- and inter-assay coefficients of variation of <3.4% and a range of analytical recovery of 98.0-103.7%. Urinary 18-hydroxycortisol excretion for APA, IHA and EH were determined as 725 (SD 451), 102 (SD 68) and 88 (SD 76) nmol/day, respectively. Conclusions: The proposed method met the basic analytical requirements and was considered to be useful in the screening and differential diagnosis of APA.
引用
收藏
页码：450 / 456
页数：7
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