Determination of Cefoperazone Based on Nano-Composite Electrode Using Coulometric FFT Admittance Voltammetry

In this work, new nano-composite modified carbon paste electrode for Cefoperazone detection is presented. Nano-composite paste was made by mixing ionic liquid, 1-butyl-3-methylimidazolium hexafluorophosphate (BMIMPF6), grapheme nanosheets, Au and CeO2 nanoparticles as electrochemical mediators. The developed electrochemical method for determination of Cefoperazone was based on coulometric fast Fourier transformation admittance voltammetry (CFFTAV). The analyte signal was based on the charge under the admittance peak, which was calculated by integrating signal in a specific potential range. The response of the unmodified carbon paste electrode (CPE) was determined and compared to the modified one (MCPE). Once established the best operative optimum conditions, the resulting nano-composite electrode showed improved sensitivities (S=53.2nC/mu M for Cefoperazone) and a good reproducibility around 97%. The response is linear in the Cefoperazone concentration range of 2 to 120x10(-9) M with a detection limit of 1.20x10(-11) M. Moreover, the proposed method exhibited high sensitivity, fast response time (less than 5 s) and long-term stability. The proposed method was successfully used in the determination of Cefoperazone content in the pharmaceutical formulation.