A highly specific ELISA for diagnosis of 2009 influenza A (H1N1) virus infections

被引：11

作者：

Lu, Chun-Yi ^{[1
]}

Chang, Luan-Yin ^{[1
]}

Chen, Pei-Jer ^{[2
]}

Xia, Ning-Shao ^{[3
]}

Shao, Pei-Lan ^{[1
]}

Huang, Li-Min ^{[1
]}

机构：

[1] Natl Taiwan Univ, Dept Pediat, Coll Med, Natl Taiwan Univ Hosp, Taipei 100, Taiwan

[2] Natl Taiwan Univ, Dept Internal Med, Natl Taiwan Univ Hosp, Coll Med, Taipei 100, Taiwan

[3] Xiamen Univ, Natl Inst Diagnost & Vaccine Dev Infect Dis, Minist Educ Cell Biol & Tumour Cell Engn, Key Lab, Xiamen, Peoples R China

来源：

JOURNAL OF THE FORMOSAN MEDICAL ASSOCIATION | 2012年 / 111卷 / 12期

关键词：

diagnosis; ELISA; pandemic; 2009 Influenza A (H1N1); TESTS; IMMUNOASSAY; SENSITIVITY; PCR;

D O I：

10.1016/j.jfma.2011.11.029

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Background/Purpose: Accurate and timely diagnosis is vital for the clinical management of influenza. A 2009 pandemic influenza A (H1N1)-specific enzyme-linked immunosorbent assay (ELISA) was tested with selected clinical samples. Methods: A selection of 90 throat swab samples with various viral loads of 2009 pandemic influenza A (H1N1) were tested. Results: Using the reverse transcriptase polymerase chain reaction as a gold standard, the overall sensitivity (0.57) was higher than that of the QuickVue Influenza A+B Test (0.43). The specificity of the ELISA was 1.0 using the selected sample set. The positive and negative predictive values were 1 and 0.4, respectively. Conclusion: The ELISA is an easy to perform, highly specific, and fairly sensitive diagnostic tool for the 2009 pandemic influenza A (H1N1) virus infections. A strong correlation was found between viral load and specificity. Copyright (C) 2012, Elsevier Taiwan LLC & Formosan Medical Association. All rights reserved.

引用

页码：693 / 697

页数：5

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