Transgenic plants over-expressing insect-specific microRNA acquire insecticidal activity against Helicoverpa armigera: an alternative to Bt-toxin technology

被引:62
|
作者
Agrawal, Aditi [1 ]
Rajamani, Vijayalakshmi [1 ]
Reddy, Vanga Siva [1 ]
Mukherjee, Sunil Kumar [2 ]
Bhatnagar, Raj K. [1 ]
机构
[1] Int Ctr Genet Engn & Biotechnol ICGEB, New Delhi 110067, India
[2] Univ Delhi, Dept Genet, New Delhi 110021, India
关键词
Artificial miRNA; Agrobacterium; Transgenic tobacco; Insecticidal; Insect bioassay; DOUBLE-STRANDED-RNA; ARTIFICIAL MICRORNAS; LARVAL RNAI; RESISTANCE; GENE; TRANSFORMATION; INTERFERENCE; SUPPRESSION; PETUNIA;
D O I
10.1007/s11248-015-9880-x
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The success of Bt transgenics in controlling predation of crops has been tempered by sporadic emergence of resistance in targeted insect larvae. Such emerging threats have prompted the search for novel insecticidal molecules that are specific and could be expressed through plants. We have resorted to small RNA-based technology for an investigative search and focused our attention to an insect-specific miRNA that interferes with the insect molting process resulting in the death of the larvae. In this study, we report the designing of a vector that produces artificial microRNA (amiR), namely amiR-24, which targets the chitinase gene of Helicoverpa armigera. This vector was used as transgene in tobacco. Northern blot and real-time analysis revealed the high level expression of amiR-24 in transgenic tobacco plants. Larvae feeding on the transgenic plants ceased to molt further and eventually died. Our results demonstrate that transgenic tobacco plants can express amiR-24 insectice specific to H. armigera.
引用
收藏
页码:791 / 801
页数:11
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  • [1] Transgenic plants over-expressing insect-specific microRNA acquire insecticidal activity against Helicoverpa armigera: an alternative to Bt-toxin technology
    Aditi Agrawal
    Vijayalakshmi Rajamani
    Vanga Siva Reddy
    Sunil Kumar Mukherjee
    Raj K. Bhatnagar
    Transgenic Research, 2015, 24 : 791 - 801