Molecular cloning of partial 14-3-3 genes in the marine sponge Hymeniacidon perleve and its role in differentiating infectious and non-infectious bacteria

被引：2

作者：

Fu WanTao ^{[1
,4
,5
]}

Zhang JuLin ^{[1
,6
]}

Zheng ChangBo ^{[7
]}

Liu Jing ^{[1
]}

An ZhongFu ^{[1
]}

Liu HongWen ^{[8
]}

Zhang Wei ^{[2
,3
,4
]}

机构：

[1] Dalian Ocean Univ, Inst Marine Sci Technol & Environm, Dalian 116023, Peoples R China

[2] Flinders Univ S Australia, Sch Med, Flinders Ctr Marine Bioprod Dev, Adelaide, SA 5042, Australia

[3] Flinders Univ S Australia, Sch Med, Dept Med Biotechnol, Adelaide, SA 5042, Australia

[4] Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China

[5] Key Lab Nearshore Marine Environm Sci & Technol L, Dalian 116023, Peoples R China

[6] Dalian Ocean Univ, Grad Sch, Dalian 116023, Peoples R China

[7] Dalian Ocean Univ, Vocat & Technol Coll, Dalian 116000, Peoples R China

[8] Dalian Environm Monitoring Ctr, Dalian 116023, Peoples R China

来源：

CHINESE SCIENCE BULLETIN | 2013年 / 58卷 / 07期

关键词：

marine sponge Hymeniacidon perleve; 14-3-3; mRNA; RT-PCR; infectious bacteria; INNATE IMMUNE DEFENSE; SUBERITES-DOMUNCULA; PROTEINS; INDUCTION; EVOLUTION; FOOD; 14-3-3-PROTEINS; INVOLVEMENT; ACTIVATION; APOPTOSIS;

D O I：

10.1007/s11434-012-5400-z

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Sponges (phylum Porifera) are the phylogenetically oldest metazoa and highly efficient filter feeders. In the marine ecosystem, they are unconditionally exposed to environmental stresses. Understanding the sponge-bacteria interaction is hence of both ecological and biological significance. This study investigated the specific interaction between the sponge Hymeniacidon perleve and the non-infectious bacteria, Escherichia coli and infectious bacteria, Vibrio spp. by measuring the 14-3-3 mRNA expression of H. perleve. Three partial cDNAs of 14-3-3 proteins and partial 18S RNA in H. perleve were cloned and sequenced. Using Reverse-transcription real-time PCR, the 14-3-3 mRNA expression of H. perleve was examined when exposed to three common bacteria in aquatic water-E. coli and two Vibrio spp. for different time and dosages. H. perleve could efficiently remove E. coli from the water column without self-infection; however Vibrio at higher dosages infected H. perleve. When H. perleve was exposed to E. coli (1.1-10(7) CFU mL(-1)), V. anguillarum II (1.2-10(6) CFU mL(-1)) and V. alginolyticus (3.6-10(5) CFU mL(-1)) for 6 h, the 14-3-3 mRNA expression in the V. anguillarum II and V. alginolyticus groups was down-regulated by 2.67- and 2.36-fold, respectively. The 14-3-3 mRNA expression in the E. coli group was not significantly different. However, no clear trend was observed on the 14-3-3 transcript levels of H. perleve in response to different doses of V. anguillarum II for different time. The results demonstrated that infectious bacteria can be discriminated by 14-3-3 mRNA expression of sponge H. perleve.

引用

页码：766 / 776

页数：11