HPLC-MS/MS analysis of 9 artificial sweeteners in imported foods

被引：31

作者：

Lim, Ho-Soo ^{[1
]}

Park, Sung-Kwan ^{[1
]}

Kwak, In-Shin ^{[1
]}

Kim, Hyung-Il ^{[1
]}

Sung, Jun-Hyun ^{[1
]}

Jang, Su-Jin ^{[1
]}

Byun, Mi-Youn ^{[1
]}

Kim, So-Hee ^{[1
]}

机构：

[1] Korea Food & Drug Adm, Food Addit & Packages Div, Food Safety Evaluat Dept, Natl Inst Food & Drug Safety Evaluat, Cheongwon 363951, Chungbuk, South Korea

来源：

FOOD SCIENCE AND BIOTECHNOLOGY | 2013年 / 22卷 / 01期

关键词：

artificial sweetener; HPLC-MS; imported food; LIQUID-CHROMATOGRAPHY; ASPARTAME;

D O I：

10.1007/s10068-013-0072-2

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

An analytical method for the simultaneous determination of 9 artificial sweeteners (acesulfame-K, cyclamate, sodium saccharin, aspartame, alitame, neotame, sucralose, dulcin, and neohesperidine dihydrochalcone) in imported foods by HPLC-MS/MS was developed. Samples were extracted with buffer solution (pH 4.5). The supernatant was analyzed by HPLC-MS/MS after centrifugation and filtration. The sample was separated on a thermo hypersil BPS C-18 (250x3 mm, 5-mu m) column, and detected by MS/MS with selective reaction monitoring (SRM) mode. The correlation coefficients (r (2)) of the calibration curve were > 0.99. The recoveries were 90.0-107.5% with good coefficients of variation of 1.8-8.6%. The limits of detection and limits of quantification were between 0.001 and 0.375 mg/mL and between 0.003 and 1.125 mg/mL, respectively. The proposed method has been successfully applied to the determination of the 9 sweeteners in various foods.

引用

页码：233 / 240

页数：8

共 22 条

[1] [Anonymous], 2004, OFF J EUR UNION L, V204, P10
[2] CHEN BH, 1995, CHROMATOGRAPHIA, V41, P43, DOI [10.1007/BF02687999, 10.1007/BF02274194]
[3] Simultaneous RP-LC determination of additives in soft drinks
Dossi, N.
Toniolo, R.
Susmel, S.
Pizzariello, A.
Bontempelli, G.
[J]. CHROMATOGRAPHIA, 2006, 63 (11-12) : 557 - 562
[4] Determination of sucralose in splenda and a sugar-free beverage using high-performance anion-exchange chromatography with pulsed amperometric detection
Hanko, VP
Rohrer, JS
[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2004, 52 (14) : 4375 - 4379
[5] Direct simultaneous determination of eight sweeteners in foods by capillary isotachophoresis
Herrmannova, Michaela
Krivankova, Ludmila
Bartos, Martin
Vytras, Karel
[J]. JOURNAL OF SEPARATION SCIENCE, 2006, 29 (08) : 1132 - 1137
[6] Rapid determination of cyclamate in foods by solid-phase extraction and capillary electrophoresis
Horie, Masao
Ishikawa, Fusako
Oishi, Mitsuo
Shindo, Tetsuya
Yasui, Akiko
Ito, Koichi
[J]. JOURNAL OF CHROMATOGRAPHY A, 2007, 1154 (1-2) : 423 - 428
[7] [Joint FAO WHO Expert Committee on Food Additives], 2004, WHO FOOD ADD SER, V52, P85
[8] Joint FAO/WHO Expert Committee on Food Additives, 2003, WHO FOOD ADDIT SER, V50, P1
[9] KFDA, 2011, FOOD ADD COD, P94
[10] Analysis of nine kinds of sweeteners in foods by LC/MS
Koyama, M
Yoshida, K
Uchibori, N
Wada, I
Akiyama, K
Sasaki, T
[J]. JOURNAL OF THE FOOD HYGIENIC SOCIETY OF JAPAN, 2005, 46 (03): : 72 - 78

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