Spatial photorelease of oligonucleotides, using a safety-catch photolabile linker

被引：13
|
作者：
Flickinger, Shane T.
Patel, Madhusudan
Binkowski, Brock F.
Lowe, Aaron M.
Li, Mo-Huang
Kim, Changhan
Cerrina, Franco
Belshaw, Peter J.
机构：
[1] Univ Wisconsin, Dept Chem, Madison, WI 53706 USA
[2] Univ Wisconsin, Dept Biochem, Madison, WI 53706 USA
[3] Univ Wisconsin, Dept Elect & Comp Engn, Madison, WI 53706 USA
[4] Univ Wisconsin, Mat Sci Program, Madison, WI 53706 USA
[5] Univ Wisconsin, Ctr Nanotechnol, Madison, WI 53706 USA
来源：
ORGANIC LETTERS | 2006年 / 8卷 / 11期
关键词：
D O I：
10.1021/ol060644x
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
We report the development of a safety-catch photolabile linker that allows the light-directed synthesis and spatially selective photorelease of oligonucleotides from microarrays. The linker remains stable to light during DNA synthesis, and is activated for photorelease after acidic hydrolysis. We demonstrate that the photoreleased oligonucleotides can be amplified by PCR to produce double stranded DNA. The advantages offered by this linker could aid the development of an automated gene synthesis platform.
引用
收藏
页码：2357 / 2360
页数：4
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