Simple Summary In recent years, the lamb production system in North-Eastern Spain has undergone an important change and the number of lamb feedlots has increased significantly. However, the possible impact of stressors associated with this production system on the health and productive performance of the lambs has not been investigated. In this study, the stress level of Rasa Aragonesa fattening lambs, finished at a commercial feedlot or on the farm of birth, was measured throughout the fattening period. Our results show that, although feedlot lambs seemed to have experienced higher stress levels than those fattened on the farm of origin, finishing location did not affect the probability of presenting clinical signs of illness and ovine respiratory complex lesions, or the final weight of the lambs. Notwithstanding, associations were found between the concentration of fecal cortisol metabolites (a chronic stress indicator) at the time of weaning and the health condition and the final weight of the lambs, regardless of the location at which they were fattened. The stress level experienced by lambs prior to finishing seems to be relevant for their health and productive performance, and measures to reduce this stress should be further investigated as a useful tool to improve the sustainability of the ovine production system. In order to determine whether the stress level had any influence on the health and final weight of Rasa Aragonesa fattening lambs, stress markers were measured throughout the fattening period in 80 feedlot lambs (group F) and in 40 lambs finished on the farm of birth (group C). The highest values of the stress indicators-neutrophil-to-lymphocyte-ratio (N/L), non-esterified fatty acids (NEFA), serum cortisol and fecal cortisol metabolites (FCM)-were recorded after the road transportation of group F to the feedlot. No differences between the groups were identified at the end of the adaptation period, but at the end of the fattening, statistically significant higher values for NEFA and serum cortisol were determined in group F. However, statistically significant differences between the groups were not found in the percentage of lambs with clinical signs of illness and ovine respiratory complex (ORC) lesions or in the final weight of the lambs. Independent of the location at which the lambs were fattened, those with FCM values at the beginning of the study (at the time of weaning) in the highest quartile developed a higher percentage of clinical signs of illness (45.5% vs. 32.1%,p> 0.05) and ORC lesions (45.5% vs. 10.7%,p< 0.01), and their average final weight was lower (24.36 kg vs. 27.40 kg,p= 0.001) than those with values in the lowest quartile. Stress experienced by lambs prior to finishing seems to be relevant for their further development, and FCM concentration at the time of weaning could be used as an indicator of health and productive performance of the lambs during the fattening period, regardless of finishing location.
