Two-photon excitation improves multifocal structured illumination microscopy in thick scattering tissue

被引：112
|
作者：
Ingaramo, Maria [1 ]
York, Andrew G. [1 ]
Wawrzusin, Peter [1 ]
Milberg, Oleg [2 ]
Hong, Amy [3 ]
Weigert, Roberto [2 ]
Shroff, Hari [1 ]
Patterson, George H. [1 ]
机构：
[1] Natl Inst Biomed Imaging & Bioengn, NIH, Bethesda, MD 20892 USA
[2] Natl Inst Dent & Craniofacial Res, NIH, Bethesda, MD 20892 USA
[3] NHLBI, NIH, Bethesda, MD 20892 USA
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2014年 / 111卷 / 14期
基金：
美国国家卫生研究院;
关键词：
multiphoton; superresolution; DIFFRACTION-LIMIT; RESOLUTION LIMIT; FLUORESCENCE; PINHOLE; ARRAY; LIVE; BREAKING; SINGLE;
D O I：
10.1073/pnas.1314447111
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Multifocal structured illumination microscopy (MSIM) provides a twofold resolution enhancement beyond the diffraction limit at sample depths up to 50 mu m, but scattered and out-of-focus light in thick samples degrades MSIM performance. Here we implement MSIM with a microlens array to enable efficient two-photon excitation. Two-photon MSIM gives resolution-doubled images with better sectioning and contrast in thick scattering samples such as Caenorhabditis elegans embryos, Drosophila melanogaster larval salivary glands, and mouse liver tissue.
引用
收藏
页码：5254 / 5259
页数：6
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