Relationship between single nucleotide polymorphisms in CYP1A1 and CYP1B1 genes and the bone mineral density and serum lipid profiles in. postmenopausal Japanese women taking hormone therapy

Objective: The genetic variations of the genes encoding cytochrome P-450 enzymes are considered to play an important role in the metabolism of estradiol. The objective of this Study was to evaluate the relationships among single nucleotide polymorphisms (SNPs) or cytochrome P-450 genes, lumbar bone mineral density (BMD), and serum lipids and to determine the effects of hormone therapy (FIT). Design: The participants were 124 Japanese women who had been diagnosed with osteopenia or osteoporosis and were taking FIT for 12 months. Seven single nucleotide polymorphisms in the CYP1A1 and CYP1B1 genes were characterized. Lumbar BMD and the levels of scrum lipids were measured before and after FIT. Results: A single nucleotide polymorphism in exon 3 of CYP1B1 was found to be significantly associated with the effect of FIT oil BMD and low-density lipoprotein cholesterol both in univariate and multivariate analyses. In the women with the GG genotype of L432V, the responses to FIT of BMD and low-density lipoprotein cholesterol markedly decreased. The serum follicle-stinulating hormone level after FIT was significantly higher ill the women with the GG genotype of L432V. Conclusions: These results suggest that the L432V polymorphism in the CYP1B1 gene could therefore be used to predict the effect of FIT oil lumbar BMD and low-density lipoprotein cholesterol in Japanese women.