Cinnamyl acetate synthesis by lipase-catalyzed transesterification in a solvent-free system

被引：26

作者：

Geng, Bo ^{[1
]}

Wang, Mengfan ^{[1
]}

Qi, Wei ^{[1
,2
,3
]}

Su, Rongxin ^{[1
,2
,3
]}

He, Zhimin ^{[1
,2
]}

机构：

[1] Tianjin Univ, Chem Engn Res Ctr, Sch Chem Engn & Technol, Tianjin 300072, Peoples R China

[2] Tianjin Univ, State Key Lab Chem Engn, Tianjin 300072, Peoples R China

[3] Tianjin Univ, Tianjin Key Lab Membrane Sci & Desalinat Technol, Tianjin 300072, Peoples R China

来源：

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY | 2012年 / 59卷 / 04期

关键词：

flavor ester; Novozym; 435; transesterification; solvent-free system; kinetic model; CANDIDA-RUGOSA LIPASE; ISOAMYL ACETATE; IMMOBILIZED-LIPASE; ENZYMATIC-SYNTHESIS; VINYL-ACETATE; ESTERIFICATION; LAURATE; ESTERS; FLAVOR; BUTYRATE;

D O I：

10.1002/bab.1023

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cinnamyl acetate was synthesized using immobilized lipase through transesterification between ethyl acetate and cinnamyl alcohol. In the solvent-free system, ethyl acetate acted as not only the acyl donor but also as the solvent of cinnamyl alcohol. Conversion (90.06%) was achieved after 3 H when transesterification was carried out at ethyl acetate/cinnamyl alcohol 15:1, 2.67 g L of lipase (Novozym 435) loading, and 40 degrees C. Excellent stability and reusability of the enzyme resulted from the moderate reaction system. Kinetic studies showed that the Michaelis constants for ethyl acetate and cinnamyl alcohol and the inhibition constant of cinnamyl alcohol were 2.241, 206.82, and 0.461 mmol L-1, respectively, which indicated that the reaction complied with the PingPong BiBi mechanism, with the inhibition of cinnamyl alcohol on lipase.

引用

页码：270 / 275

页数：6

共 50 条

[1] Lipase-catalyzed transesterification synthesis of citronellyl acetate in a solvent-free system and its reaction kinetics
Jian Xiong
Ya-Juan Huang
Hua Zhang
European Food Research and Technology, 2012, 235 : 907 - 914
[2] Lipase-catalyzed transesterification synthesis of citronellyl acetate in a solvent-free system and its reaction kinetics
Xiong, Jian
Huang, Ya-Juan
Zhang, Hua
EUROPEAN FOOD RESEARCH AND TECHNOLOGY, 2012, 235 (05) : 907 - 914
[3] Lipase-catalyzed solvent-free transesterification of wood sterols
Martínez, I
Markovits, A
Chamy, R
Markovits, A
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2004, 112 (01) : 55 - 62
[4] Lipase-catalyzed solvent-free transesterification of wood sterols
Irene Martínez
Alejandro Markovits
Rolando Chamy
Andrés Markovits
Applied Biochemistry and Biotechnology, 2004, 112 : 55 - 62
[5] OPTIMIZATION OF LIPASE-CATALYZED SYNTHESIS OF CITRONELLYL ACETATE IN SOLVENT-FREE MEDIUM
FONTEYN, F
BLECKER, C
LOGNAY, G
MARLIER, M
SEVERIN, M
BIOTECHNOLOGY LETTERS, 1994, 16 (07) : 693 - 696
[6] LIPASE-CATALYZED SYNTHESIS OF LYSOPHOSPHATIDIC ACID IN A SOLVENT-FREE SYSTEM
HAN, JJ
RHEE, JS
BIOTECHNOLOGY LETTERS, 1995, 17 (05) : 531 - 536
[7] Lipase-catalyzed transesterification of ethyl ferulate with triolein in solvent-free medium
Xin, Jia-ying
Chen, Lin-lin
Zhang, Ying-xin
Zhang, Shuai
Xia, Chun-gu
FOOD AND BIOPRODUCTS PROCESSING, 2011, 89 (C4) : 457 - 462
[8] Ultrasound assisted lipase catalyzed synthesis of cinnamyl acetate via transesterification reaction in a solvent free medium
Tomke, Prerana D.
Rathod, Virendra K.
ULTRASONICS SONOCHEMISTRY, 2015, 27 : 241 - 246
[9] Monoglycerides and diglycerides synthesis in a solvent-free system by lipase-catalyzed glycerolysis
Lucente Fregolente, Patricia Bogalhos
Fregolente, Leonardo Vasconcelos
Pinto, Glaucia Maria F.
Batistella, Benedito Cesar
Wolf-Maciel, Maria Regina
Maciel Filho, Rubens
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2008, 146 (1-3) : 165 - 172
[10] Synthesis of Eugenol Esters by Lipase-Catalyzed Reaction in Solvent-Free System
Chiaradia, V.
Paroul, N.
Cansian, R. L.
Junior, C. V.
Detofol, M. R.
Lerin, L. A.
Vladimir Oliveira, J.
Oliveira, D.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2012, 168 (04) : 742 - 751

← 1 2 3 4 5 →