It is not long ago that e-commerce service has turned to be one of the most important applications of the Internet and www. In all aspects of human life, especially in business activity, the application of ICT is so strong that in near future, the world will witness inevitability of this technology in most of the daily issues. Regardless of its technological advantages in the areas of increasing speed, accuracy, ease and dramatic reduction of transaction or processing costs, many challenges and application barriers are continuously faced by its users. Concerns such as users' privacy breaches, ensuring the accuracy, original identification of the buyers and sellers, confidentiality provision cause discomfort and distress an individual during cyberspace transaction or deal. And the achievement of the desired level of confidence in the abilities and capabilities of the other side and performance endanger the system. The increased level of awareness and the security culture of the cyberspace application enhance relative peace of an individual while being in the virtual space or in the process of e-commerce. The resulting comfort helps a person to identify and belief in the capabilities of the opposite side or system and their adherence to the commercial, behavioral, legal and technical principles connected to right deals. In cyberspace under discussion, this identification or situation is corresponding with electronic trust. E-Trust is the pillar of E-commerce. This article will discuss the role of e-commerce awareness in increasing the electronic trust. [Issa Najafi. The Role of e-Commerce Awareness on Increasing Electronic Trust. Life Sci J 2012;9(4):1487-1494] (ISSN:1097-8135). http://www.lifesciencesite.com. 226