Proteome Analysis of Wheat Roots at Different Potassium Level

To realize the role of potassium on the gene expression of plant, the proteome at different potassium level in wheat roots were explored by two-dimensional electrophoresis (2-DE). The wheat seedling with 3 leaves which were grown at Hogland solution were separated into two groups the first group was kept at normal Hogland solution till the end of the experiments, and the second was grown at the Hogland solution without potassium. As soon as the deficient potassium symptom of the wheat plants appeared, half of them were harvested and at the same time some plants of the first group as a comparable sample was also harvested. The other half of the plants of the second group were supplied with normal Hogland solution for one week and then harvested, at the same time the remaining plants in the first group were also harvested. The protein of every sample was only abstracted from the roots. After the proteins separated by 2-DE, the protein spots were analyzed by PDQuest software. The result sowed that comparable to the plants supplied with normal potassium level, the difference of 152 protein spots from the treated wheat roots appeared significant level (p<0.05), 76 protein spots were up-regulated by more than two times, and other 76 protein spots appeared down-regulated by more than 2-time. After the potassium deficient plants were supplied with normal potassium level for one week, comparable to the normal plants, the difference of 73 protein spots appeared significant level (p<0.05), and 36 of them were up regulated by more than 2-time, 37 spots were down-regulated by more than 2 times.