miR-671-5p Inhibits Tumor Proliferation by Blocking Cell Cycle in Osteosarcoma

被引:45
|
作者
Xin, Chaofei [1 ]
Lu, Shitao [1 ]
Li, Yu [1 ]
Zhang, Yi [1 ]
Tian, Jinxiang [1 ]
Zhang, Shaokun [1 ]
Yang, Shangliang [1 ]
Gao, Tianhao [1 ]
Xu, Jianzhong [1 ]
机构
[1] Zhengzhou Univ, Affiliated Hosp 1, Dept Orthoped Surg, Zhengzhou 450000, Henan, Peoples R China
基金
中国国家自然科学基金;
关键词
miR-671-5p; proliferation; cell cycle; osteosarcoma; CCND1; CDC34; TARGETING MICRORNAS; PROMOTES; EXPRESSION; CANCER;
D O I
10.1089/dna.2019.4870
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Osteosarcoma (OS), a highly aggressive bone tumor, mainly occurs in young patients and always presents abnormalities in molecular biology, such as microRNAs (miRNAs). However, the characteristic and underlying mechanism of miR-671-5p in OS are still unclear. In this study, we certify that miR-671-5p is remarkably downregulated in OS tissues and cells. Overexpressed miR-671-5p can suppress OS cell proliferation in vivo and in vitro, by the way of arresting cell-cycle progression. The overexpression of cyclin D1 (CCND1) and CDC34 promotes cell proliferation and cell-cycle promotion, whose functions are contrary to miR-671-5p. miR-671-5p directly binds to CCND1 and CDC34, which are thought as the key factors in regulating cell cycle. Taken together, our results suggest that by targeting CCND1 and CDC34, miR-671-5p plays a tumor suppressor in OS to inhibit the development of OS, implicating it as a novel target for therapeutic intervention in OS.
引用
收藏
页码:996 / 1004
页数:9
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