Collision cross sections of high-mannose N-glycans in commonly observed adduct states - identification of gas-phase conformers unique to [M - H]- ions

被引：36

作者：

Struwe, W. B. ^{[1
]}

Benesch, J. L. ^{[1
]}

Harvey, D. J. ^{[2
]}

Pagel, K. ^{[3
,4
]}

机构：

[1] Univ Oxford, Dept Chem, Oxford OX1 3TA, England

[2] Univ Oxford, Dept Biochem, Oxford Glycobiol Inst, Oxford OX1 3QU, England

[3] Max Planck Gesell, Fritz Haber Inst, D-14195 Berlin, Germany

[4] Free Univ Berlin, Inst Chem & Biochem, D-14195 Berlin, Germany

来源：

ANALYST | 2015年 / 14卷 / 20期

基金：

英国生物技术与生命科学研究理事会;

关键词：

MOBILITY-MASS-SPECTROMETRY; LINKED GLYCANS; FRAGMENTATION; DATABASE; MS;

D O I：

10.1039/c5an01092f

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We report collision cross sections (CCS) of high-mannose N-glycans as [M + Na](+), [M + K](+), [M + H](+), [M + Cl](-), [M + H2PO4](-) and [M -H](-) ions, measured by drift tube (DT) ion mobility-mass spectrometry (IM-MS) in helium and nitrogen gases. Further analysis using traveling wave (TW) IM-MS reveal the existence of distinct conformers exclusive to [M -H](-) ions.

引用

页码：6799 / 6803

页数：5