Emodin attenuates cell injury and inflammation in pancreatic acinar AR42J cells

被引：16
|
作者：
Zhao, Jia-Yu [1 ,2 ]
Wang, Jia-Qi [1 ]
Wu, Li [1 ,2 ]
Zhang, Feng [1 ,2 ]
Chen, Zhi-Peng [1 ,2 ]
Li, Wei-Dong [1 ,2 ]
Cai, Hao [1 ,2 ]
Liu, Xiao [1 ,2 ]
机构：
[1] Nanjing Univ Chinese Med, Coll Pharm, Dept Pharmacol, Nanjing 210023, Jiangsu, Peoples R China
[2] Nanjing Univ Chinese Med, Natl First Class Key Discipline Tradit Chinese Me, Nanjing 210023, Jiangsu, Peoples R China
来源：
JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH | 2019年 / 21卷 / 02期
基金：
中国国家自然科学基金;
关键词：
Rhubarb; emodin; acute pancreatitis; ROS; cytokine; TNF-ALPHA; INHIBITION; ACTIVATION; NECROSIS; STRESS; RATS;
D O I：
10.1080/10286020.2017.1408594
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
The study was intended to investigate the protective effects of emodin against cell injury and inflammation in AR42J cells. We determined trypsin and lipase activity, intracellular ROS and MMP using specific assay kits. The related protein expression and TNF-alpha and IL-6 in the medium were assayed by Western blot and ELISA kits. Results showed that emodin could protect AR42J cells against cell injury caused by cerulein and lipopolysaccharide which were possibly associated with inhibition of mitochondrial damage, ROS production, and then significantly inhibited ROS-mediated pathway, and ameliorated pancreatic cells injury by depleting the levels of inflammatory cytokines. [GRAPHICS] .
引用
收藏
页码：186 / 195
页数：10
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