Redox regulation of lipopolysaccharide (LPS)-induced interleukin-8 (IL-8) gene expression mediated by NF kappa B and AP-1 in human astrocytoma U373 cells

被引：77
|
作者：
Tanaka, C [1 ]
Kamata, H [1 ]
Takeshita, H [1 ]
Yagisawa, H [1 ]
Hirata, H [1 ]
机构：
[1] HIMEJI INST TECHNOL, FAC SCI, DEPT LIFE SCI, KAMIGORI CHOU, HYOGO 67812, JAPAN
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1997年 / 232卷 / 02期
关键词：
D O I：
10.1006/bbrc.1997.6264
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
LPS-induced expression of the IL-8 gene was markedly enhanced by H2O2 or by deprivation of the cellular antioxidant glutathione by L-buthionine-(S,R)-sulfoximine (BSO) in human astrocytoma U373 cells. In contrast, it was markedly suppressed by the reductant N-acetyl-L-cysteine (NAC) and other antioxidants. Transient expression analysis using the chloramphenicol acetyltransferase assay revealed that activation of the IL-8 promoter by LPS was stimulated by BSO and was suppressed by NAC; likewise LPS-induced activation of both NF kappa B and AP-1 was enhanced by BSO and inhibited by NAG. These results suggest that LPS-induced IL-8 gene expression is regulated by cellular redox via modulation of these transcription factors. (C) 1997 Academic Press.
引用
收藏
页码：568 / 573
页数：6
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