Rapid Identification of Pseudomonas aeruginosa and Pseudomonas fluorescens Using Raman Spectroscopy

Rapid and accurate identification of Pseudomonas species, especially in drinking water, is of great importance for human health. In the present study, we aim to develop a fast and reliable verification method, which does not require intensive microbiological procedures. In this method, specific Raman spectra of Pseudomonas aeruginosa, Pseudomonas fluorescens and Burkholderia pseudomallei were collected directly from the medium and principal component analysis was used for the data mining process of collected Raman spectra. Application of Raman spectroscopy accompanied with principal component analysis enabled us to differentiate the two most dominant accompanying flora among pathogen Pseudomonas species. Based on Raman spectroscopy, an alternative cultivation-free verification method with 15min analysis time was developed. It is envisioned that using this method will also contribute to prevent the unnecessary closure of water wells. Practical ApplicationsIt is well known that Pseudomonas sp. is commonly found in water and their detection is significant in terms of human health. In the present study, a rapid and cultivation-free verification method has been developed for the identification and differentiation of Pseudomonas sp. namely, Pseudomonasaeruginosa, Pseudomonasfluorescens and Burkholderiapseudomallei. The results of the study offer that Raman spectroscopy coupled with principal component analysis seems to be a simple alternative verification method with a shortened analysis time as low as 15min.