Subduing the Hepatotoxic and Urotoxic Effects of Cyclophosphamide using Natural Antioxidants

Cyclophosphamide is commercially popular chemotherapeutic drug and is used in the treatment of malignant and,non-malignant forms of cancer.:Action of hepatic microsomal enzymes on cyclophosphamide metabolizes it into phosphoramide mustard and acrolein These; are toxic and are majorly found to have ad-Verse effects on the kidney and liver causing symptoms like dypsia; fibrosis; hemorrhagic:;1 cystitis and necropsis. Acrolein one of the metabolites was found to-interact with majority of biomolecules like DNA, RNA and proteins that may lead to apoptosis. Cyclophosphamide metabolism was Also known:to induce the production of reactive oxygen species leading to total cellular damage, which causes various acute and,chronic diseases, Herbal plants are found to bp rich in antioxidant and have anticancerous and tumor reducing properties, Present study aimed in achieving modulatory action of the poly-herbal combination :a BC and Vitamin E on cyclophosphamide induced hepato- and uro-toxicity. Wistar rats (male and female) were treated orally-with the 10(1 mg/kg of the BC and 50 mg/kg of Vit-E for whole of the study (30 days). 200 mg/kg of cyclophosphamide was administered does through on day 20 and 21. Serum was used to analyze the clinical chemistry parameters such as total protein, albumin, (total and direct); urea; creatinine and enzymes like AST and ALT. Parameters that were significantly altered due to cyclophosphamide, administration were modulated during treatment using BC + Vit-E (BCE). By these preliminary studies, it has been hypothesized that the effects of cyclophosphamide were treated to a great extent and therapeutic effect of cyclophosphamide has been sensitized by BCE.