Engineering Cell Surfaces for Orthogonal Selectability

被引：4
|
作者：
Peng, Yingjie [1 ]
Kim, Dae Hee [2 ]
Jones, Teresa M. [1 ]
Ruiz, Diana I. [1 ]
Lerner, Richard A. [1 ]
机构：
[1] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA
[2] Scripps Korea Antibody Inst, Chuncheon Si 200701, Gangwon Do, South Korea
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2013年 / 52卷 / 01期
关键词：
cell selection; chitin-binding domain; gene expression; protein engineering; transfection; GREEN FLUORESCENT PROTEIN; PURIFICATION; MUTANTS;
D O I：
10.1002/anie.201201844
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Vectors have been constructed that express the chitin-binding domain (ChBD) on eukaryotic cell surfaces. The ChBD is linked to enhanced green fluorescent protein (EGFP) through a protein that spans the plasma membrane (see picture). This binding functionality does not have a counterpart in eukaryotes, thereby endowing the modified cell surface with a property that is orthogonal to animal cells. © 2013 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
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收藏
页码：336 / 340
页数：5
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