Antigenotoxic effect of green-synthesised silver nanoparticles from Ocimum sanctum leaf extract against cyclophosphamide induced genotoxicity in human lymphocytes-in vitro

The present study was aimed to identify the antigenotoxic effect of bio-synthesised silver nanoparticles (SNP) of Ocimum sanctum leaf extract against cyclophosphamide (CP). We tested the antigenotoxic effect of bio-synthesized silver nanoparticles of O. sanctum leaf extract on human lymphocytes against CP by using chromosomal aberration assay (CAA). Silver nanoparticles was first synthesized from fresh leaf extract of O. sanctum and characterised. Their quality was checked by XRD technique and morphology by SEM. Three different doses of the bio-synthesised SNPs namely, 50, 100 and 200 mu l/ml were selected and CP (100 mu g/ml) was used as a positive control for CAA. CP administration to human lymphocytes culture caused reduction in mitotic index (MI) and increase in chromosomal damages. The three doses (50, 100 and 200 mu l/ml) significantly (P < 0.005) reduced the chromosomal damages by CP and there was increase in MI. The biological way of synthesising SNPs has advantages like cost effectiveness and eco-friendly. Also the bio-synthesised SNPs of O. sanctum leaf extract was found to be an powerful genoprotectant. Furthermore works are to be carried out in future to find the extract mechanism of its genoprotective nature.