Mycobacterium bovis identification by a molecular method from post-mortem inspected cattle obtained in abattoirs of Mato Grosso do Sul, Brazil

被引：20
|
作者：
de Araújo, CP
Leite, CQF
de Prince, KA
Jorge, KDG
Osório, ALAR
机构：
[1] Univ Fed Mato Grosso do Sul, BR-79070900 Campo Grande, MS, Brazil
[2] Univ Estadual Paulista, Fac Ciencias Farmaceut, Araraquara, SP, Brazil
来源：
MEMORIAS DO INSTITUTO OSWALDO CRUZ | 2005年 / 100卷 / 07期
关键词：
Mycobacterium bovis; nontuberculous mycobacteria; polymerase chain reaction; PCR-restriction enzyme pattern analysis; cattle; post-mortem inspection; abattoirs;
D O I：
10.1590/S0074-02762005000700013
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
The presence of Mycobacterium bovis in bovine carcasses with lesions suggestive of tuberculosis was evaluated. Seventy-two carcass samples were selected during slaughter inspection procedures in abattoirs in the state of Mato Grosso do Sul, Brazil. Seventeen (23.6%) of samples showed colonies suggestive of mycobacteria that were confirmed to be acid-fast bacilli by Ziehl-Neelsen staining. Polymerase chain reaction (PCR) using primers specific for M. bovis identified M. bovis in 13 (76.5%) isolates. The PCR-restriction enzyme pattern analysis using gene encoding for the 65-kDa protein and two restriction enzymes identified the remaining four isolates that were represented by two M. tuberculosis complex and two nontuberculous mycobacteria. The results are indicative of infection of slaughter cattle by M. bovis and other mycobacteria in the state of Mato Grosso do Sul.
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收藏
页码：749 / 752
页数：4
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